{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:21:07Z","timestamp":1787016067446,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFF1003502"],"award-info":[{"award-number":["2022YFF1003502"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470352"],"award-info":[{"award-number":["32470352"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Nexine is an essential layer of the pollen wall. TRANSPOSABLE ELEMENT SILENCING VIA AT\u2010HOOK (TEK), a member of the AT\u2010HOOK MOTIF CONTAINING NUCLEAR\u2010LOCALIZED (AHL) family, is a key regulator for nexine formation in Arabidopsis. In this study, we report that TEK interacts with TEOSINITE BRANCHED1\u2010CYCLOIDEA\u2010PCF 14 (TCP14) and TCP15 to regulate nexine formation. TCP14\/15 are expressed in the anther cell layers including the tapetum. Although\n                    <jats:italic>tcp14 tcp15<\/jats:italic>\n                    has no anther development defect, specifically repressing TCP15 and its homologs during the nexine formation stage in the\n                    <jats:italic>pAMS::TCP15\u2010SRDX<\/jats:italic>\n                    transgenic plants leads to a defect in the nexine layer and complete sterility. Cell wall and other pollen wall formation\u2010related genes are significantly enriched among downregulated genes in the transgenic line. This indicates that TCP14\/15 and other TCP members are required for nexine formation. In addition,\n                    <jats:italic>tek<\/jats:italic>\n                    exhibits thermosensitive genic male sterility (TGMS) as its fertility was partially restored under low temperature (17\u00b0C), suggesting other AHL family members play a redundant role in nexine formation. AHL8 is localized in the tapetum and interacts with TCP14 and TCP15. Although the fertility of the\n                    <jats:italic>ahl8 tek<\/jats:italic>\n                    double mutant is similar to\n                    <jats:italic>tek<\/jats:italic>\n                    under 17\u00b0C, dominant suppression of AHL homologs in\n                    <jats:italic>pAMS::AHL8\u2010SRDX<\/jats:italic>\n                    transgenic plants exhibits complete male sterility under 17\u00b0C. All these indicate that AHL8 and other AHLs play a redundant role in interacting with TCPs to regulate nexine formation. This work not only reveals the regulatory mechanism for nexine formation, but also provides a novel thermosensitive genic male sterile (TGMS) locus that may have potential applications in agriculture.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70583","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T07:42:47Z","timestamp":1763451767000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>TEK<\/scp>\n                    and other\n                    <scp>AHL<\/scp>\n                    proteins interact with\n                    <scp>TCP14<\/scp>\n                    \/15 to regulate pollen wall formation in Arabidopsis"],"prefix":"10.1111","volume":"124","author":[{"given":"Shuang\u2010Xi","family":"Xiong","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Plant Molecular Sciences College of Life Sciences Shanghai Normal University  Shanghai 200234 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