{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:02:33Z","timestamp":1790697753066,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T00:00:00Z","timestamp":1764979200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K06251"],"award-info":[{"award-number":["21K06251"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K06284"],"award-info":[{"award-number":["22K06284"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K09677"],"award-info":[{"award-number":["25K09677"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31560065"],"award-info":[{"award-number":["31560065"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31860216"],"award-info":[{"award-number":["31860216"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32260403"],"award-info":[{"award-number":["32260403"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia Autonomous Region","doi-asserted-by":"publisher","award":["2017MS0329"],"award-info":[{"award-number":["2017MS0329"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia Autonomous Region","doi-asserted-by":"publisher","award":["2025MS03056"],"award-info":[{"award-number":["2025MS03056"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    It is understood that a cyanobacterium was the progenitor of plastids and that the biosynthesis of cell wall peptidoglycan was lost during chloroplast evolution. However, accumulated data, especially from the moss\n                    <jats:italic>Physcomitrium patens<\/jats:italic>\n                    , suggest that peptidoglycan remains essential for plastid division in some land plants. A fundamental set of peptidoglycan biosynthesis (\n                    <jats:italic>Mur<\/jats:italic>\n                    ) genes has been identified in the genomes of these land plants, while many angiosperms no longer encode some core\n                    <jats:italic>Mur<\/jats:italic>\n                    genes, including a bifunctional penicillin\u2010binding protein (PBP). Ten incomplete\n                    <jats:italic>Mur<\/jats:italic>\n                    genes were previously identified in the genome of the gymnosperm\n                    <jats:italic>Picea abies<\/jats:italic>\n                    but these could be pseudogenes or encode proteins that have been repurposed. For instance, mutant albino maize and Arabidopsis seedlings possess a defective UDP\u2010N\u2010acetylmuramoyl\u2010\n                    <jats:sc>l<\/jats:sc>\n                    \u2010alanyl\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010glutamate\u2010\u20102,6\u2010diaminopimelate ligase (MurE), an intact MurE ligase being essential for peptidoglycan synthesis. In this study, we isolated a full set of cDNAs for peptidoglycan biosynthesis from\n                    <jats:italic>P. abies<\/jats:italic>\n                    . GFP fusion proteins with either\n                    <jats:italic>P. abies<\/jats:italic>\n                    (Pa)MurE or PaPBP were detected in chloroplasts. Cross\u2010species complementation assays with\n                    <jats:italic>PaMurE<\/jats:italic>\n                    in Arabidopsis albino\n                    <jats:italic>MurE<\/jats:italic>\n                    mutants and Physcomitrium\n                    <jats:italic>MurE<\/jats:italic>\n                    chloroplast division mutants showed that the gymnosperm\n                    <jats:italic>MurE<\/jats:italic>\n                    completely rescued both mutant phenotypes. Enzymatic assay of recombinant PaMurE proteins revealed they catalyze the same reaction performed by their bacterial MurE homologs. Moreover, the expression of the\n                    <jats:italic>PaPbp<\/jats:italic>\n                    cDNA partially rescued the giant chloroplast phenotype in the moss\n                    <jats:italic>Pbp<\/jats:italic>\n                    knockout line. These results are consistent with the operation of a functional\n                    <jats:italic>Mur<\/jats:italic>\n                    gene set in the Norway spruce genome.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70588","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T22:19:47Z","timestamp":1765059587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptomic and enzymological evidence for plastid peptidoglycan synthesis in the gymnosperm\n                    <i>Picea abies<\/i>"],"prefix":"10.1111","volume":"124","author":[{"given":"Yayoi","family":"Sugita","sequence":"first","affiliation":[{"name":"Graduate School of Science and Technology Kumamoto University  Kumamoto 860\u20108555 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1813-603X","authenticated-orcid":false,"given":"Amanda J.","family":"Dowson","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Warwick  Coventry CV4 7AL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Kajisa","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Technology Kumamoto University  Kumamoto 860\u20108555 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuaki","family":"Takechi","sequence":"additional","affiliation":[{"name":"Faculty of Advanced Science and Technology Kumamoto University  Kumamoto 860\u20108555 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilan","family":"E","sequence":"additional","affiliation":[{"name":"College of Life Sciences Inner Mongolia University  Hohhot 010021 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingzhi","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Sciences Inner Mongolia University  Hohhot 010021 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences Inner Mongolia University  Hohhot 010021 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Life Sciences Inner Mongolia University  Hohhot 010021 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Diaz\u2010Saez","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Warwick  Coventry CV4 7AL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian J.","family":"Lloyd","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Warwick  Coventry CV4 7AL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher G.","family":"Dowson","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Warwick  Coventry CV4 7AL UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4720-5057","authenticated-orcid":false,"given":"Hiroyoshi","family":"Takano","sequence":"additional","affiliation":[{"name":"Faculty of Advanced Science and Technology Kumamoto University  Kumamoto 860\u20108555 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00265581"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm047"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2020.112370"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444903027720"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505622200"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19268"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00483-9"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.117.040055"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.751891"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2024.02.003"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19393"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac176"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10761.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-024-10929-4"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-306-48568-8_7"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03379.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr944"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.16.00104"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.5.1191-1199.2000"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcp158"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24773-7"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.072629"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1529-8817.2003.02132.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/8.3.275"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0176-1617(97)80193-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-04715-5"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077356"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcx005"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-008-0762-9"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510693103"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-023-01886-y"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jse.12516"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.14.4321-4327.1994"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.119.400083"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-95547-w"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.117.300078"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkab380"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.2.332-339.1996"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-021-03143-x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1150646"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.097444"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-008-0872-4"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-017-0935-3"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcx113"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1515\/znb-1970-0627"},{"key":"e_1_2_9_49_1","first-page":"1952","article-title":"The western redcedar genome reveals low genetic diversity in a self\u2010compatible conifer","volume":"32","author":"Shalev T.J.","year":"2022","journal-title":"Genome Research"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz312"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13265"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13557"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.92.499"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2009.07.020"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac278"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12040563"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-020-01081-0"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-2585-6"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/11.3.25R"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.01.036"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giac078"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-017-0097-2"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-54266-2"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.228726"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.01.026"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-021-00963-5"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.199"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giw016"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.70588","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tpj.70588","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.70588","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:07:20Z","timestamp":1790694440000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.70588"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":68,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/tpj.70588"],"URL":"https:\/\/doi.org\/10.1111\/tpj.70588","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2025-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70588"}}