{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:02:05Z","timestamp":1790697725706,"version":"4.1.0"},"reference-count":92,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022MC031"],"award-info":[{"award-number":["ZR2022MC031"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    As the last stage of leaf development, senescence is orchestrated by an intricate network of endogenous factors and external signals to ensure an efficient recycling of nutrients. Hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) serves as an important gaseous signaling molecule in plants, mediating a myriad of physiological processes like leaf senescence. However, the molecular mechanisms underlying H\n                    <jats:sub>2<\/jats:sub>\n                    S accumulation and its regulation during leaf senescence in tobacco are still not fully elucidated. In this work, we demonstrate that NtWRKY75, a WRKY transcription factor in tobacco (\n                    <jats:italic>Nicotiana tabacum<\/jats:italic>\n                    ), serves as a negative regulator of the expression of the key genes involved in H\n                    <jats:sub>2<\/jats:sub>\n                    S biosynthesis (\n                    <jats:sc>l<\/jats:sc>\n                    \u2010\n                    <jats:italic>cysteine desulfhydrase<\/jats:italic>\n                    ,\n                    <jats:italic>NtLCD1<\/jats:italic>\n                    ;\n                    <jats:sc>d<\/jats:sc>\n                    \u2010\n                    <jats:italic>cysteine desulfhydrase<\/jats:italic>\n                    ,\n                    <jats:italic>NtDCD1<\/jats:italic>\n                    ), thereby accelerating dark\u2010induced leaf senescence. The transcript levels of\n                    <jats:italic>NtWRKY75<\/jats:italic>\n                    are progressively upregulated during both dark\u2010induced and natural leaf senescence. Transgenic tobacco plants overexpressing\n                    <jats:italic>NtWRKY75<\/jats:italic>\n                    show premature leaf senescence, while\n                    <jats:italic>ntwrky75<\/jats:italic>\n                    mutants generated through CRISPR\/Cas9 exhibit delayed leaf senescence. Further molecular and biochemical analyses reveal that senescence\u2010associated NtWRKY75 binds to the promoters of\n                    <jats:italic>NtDCD1<\/jats:italic>\n                    and\n                    <jats:italic>NtOASA1<\/jats:italic>\n                    , downregulating\n                    <jats:italic>NtDCD1<\/jats:italic>\n                    expression while upregulating\n                    <jats:italic>NtOASA1<\/jats:italic>\n                    , thereby leading to decreased H\n                    <jats:sub>2<\/jats:sub>\n                    S accumulation. NtWRKY75 also interacts with the promoters of multiple amino acid transporter genes, including\n                    <jats:italic>NtAAP3<\/jats:italic>\n                    , resulting in their upregulation and facilitating amino acid remobilization, which accelerates leaf senescence. Additionally, NtVQ47, a protein containing the VQ motif, physically interacts with NtWRKY75\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    , thereby fine\u2010tuning its transcriptional activity and influencing leaf senescence. In conclusion, our findings demonstrate that the regulatory network composed of NtVQ47, NtWRKY75, and H\n                    <jats:sub>2<\/jats:sub>\n                    S plays a crucial role in precisely modulating leaf senescence, offering promising candidates and strategies for future crop improvement.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70616","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T08:50:26Z","timestamp":1764838226000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcription factor\n                    <scp>NtWRKY75<\/scp>\n                    accelerates dark\u2010induced leaf senescence through orchestrating hydrogen sulfide homeostasis in\n                    <i>Nicotiana tabacum<\/i>"],"prefix":"10.1111","volume":"124","author":[{"given":"Songchong","family":"Lu","sequence":"first","affiliation":[{"name":"Shandong Key Laboratory for Germplasm Innovation of Saline\u2010Alkaline Tolerant Grasses and Trees, College of Grassland Science Qingdao Agricultural University  Qingdao 266109 China"},{"name":"College of Life Sciences Qingdao Agricultural University  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"Shandong Key Laboratory for Germplasm Innovation of Saline\u2010Alkaline Tolerant Grasses and Trees, College of Grassland Science Qingdao Agricultural University  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences Qingdao Agricultural University  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences Qingdao Agricultural University  Qingdao 266109 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