{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:59:23Z","timestamp":1790787563552,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012542","name":"Sichuan Provincial Science and Technology Support Program","doi-asserted-by":"publisher","award":["2024ZHCG0155"],"award-info":[{"award-number":["2024ZHCG0155"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Long\u2010term domestication and cultivation have led to the differentiation of\n                    <jats:italic>Salvia miltiorrhiza<\/jats:italic>\n                    genetic resources in different regions of China, accompanied by differences in the accumulation of pharmacological components represented by tanshinones in the roots. Tanshinone, as a key biomarker with significant therapeutic effects on cardiovascular and cerebrovascular diseases, has become the primary target for improvement in\n                    <jats:italic>S. miltiorrhiza<\/jats:italic>\n                    breeding. It is particularly important to deeply analyze the differentiation of tanshinones biosynthetic pathways in\n                    <jats:italic>S. miltiorrhiza<\/jats:italic>\n                    of different ecotypes, systematically revealing the evolution mechanism of geoherbs driven by metabolic networks. Based on integrated omics strategies, the metabolic regulatory network of tanshinones was constructed in different ecotypes, and WRKY70 was identified as a core transcription factor in the regulatory network of tanshinones metabolism. Transgene, Yeast\u2010one\u2010hybrid, EMSA, and Dual\u2010LUC demonstrated that WRKY70 positively regulates tanshinones biosynthesis by targeting the promoter of\n                    <jats:italic>CYP76AK1<\/jats:italic>\n                    and\n                    <jats:italic>ABCG1<\/jats:italic>\n                    . Yeast\u2010two\u2010hybrid, BiFC, Luciferase complementation, and Co\u2010IP confirmed that WRKY70 formed a stable protein complex with SnRK2.6, which in turn mediated the ABA signaling pathway dependent tanshinones metabolic regulation and drought stress adaptation. Overall, we report that a bifunctional transcription factor, WRKY70, promotes tanshinones synthesis while enhancing plant adaptation to drought, which is expected to be used as a breeding target to save the deficiency of tanshinones and enhance stress resistance in specific ecotype\n                    <jats:italic>S. miltiorrhiza<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/tpj.70790","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:39:43Z","timestamp":1773794383000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The dual\u2010function protein complex\n                    <scp>SnRK2<\/scp>\n                    .6\u2010\n                    <scp>WRKY70<\/scp>\n                    synergistically enhances the tanshinones biosynthesis and drought stress adaptation in\n                    <i>Salvia miltiorrhiza<\/i>"],"prefix":"10.1111","volume":"125","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8889-0324","authenticated-orcid":false,"given":"Haomiao","family":"Yu","sequence":"first","affiliation":[{"name":"College of Science Sichuan Agricultural University  Ya'an 625000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxin","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Science Sichuan Agricultural University  Ya'an 625000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlin","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Science Sichuan Agricultural University  Ya'an 625000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuexue","family":"Deng","sequence":"additional","affiliation":[{"name":"College of Science Sichuan Agricultural University  Ya'an 625000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Science Sichuan Agricultural University  Ya'an 625000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Science Sichuan Agricultural University  Ya'an 625000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiwu","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Science Sichuan Agricultural University  Ya'an 625014 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2651-4777","authenticated-orcid":false,"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Science Sichuan Agricultural University  Ya'an 625000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2012.03.021"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erad498"},{"key":"e_1_2_9_4_1","first-page":"1","article-title":"Near\u2010optimal probabilistic RNA\u2010Seq quantification","volume":"34","author":"Bray N.","year":"2016","journal-title":"Nature Biotechnology"},{"key":"e_1_2_9_5_1","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Commission, C.P","year":"2020"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-191"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.10.038"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab050"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.15212"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.621169"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2011.02426.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhac238"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.04.015"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13806"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110599"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2978"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2978"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-014-1592-9"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2022.103884"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202415106"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.113950"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2022.2036521"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-024-01967-5"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-20959-1"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.049"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(19)30086-X"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.14543"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhad069"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti610"},{"key":"e_1_2_9_31_1","first-page":"1","article-title":"Traditional herbal medicines to overcome stress, anxiety and improve mental health in outbreaks of human coronaviruses","volume":"2020","author":"Shahrajabian M.H.","year":"2020","journal-title":"Phytotherapy Research"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/tpg2.20041"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70029"},{"key":"e_1_2_9_35_1","first-page":"43","volume-title":"Advances in Pharmacology","author":"Wang X.","year":"2020"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1224856"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.572373"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr483"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2016.03.010"},{"key":"e_1_2_9_40_1","first-page":"653","article-title":"Effects of salvianolic acid B on growth inhibition and apoptosis induction of ovarian cancer SKOV3","volume":"37","author":"Yan F.","year":"2016","journal-title":"European Journal of Gynaecological Oncology"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70234"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.70006"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13007-022-00872-4"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41657-0"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70048"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.3c02908"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.11326"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1484688"},{"key":"e_1_2_9_49_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2019\/3281260","article-title":"Current progress of research on neurodegenerative diseases of salvianolic acid B","volume":"2019","author":"Zhao R.","year":"2019","journal-title":"Oxidative Medicine and Cellular Longevity"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-024-05006-7"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.70790","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tpj.70790","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.70790","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T01:49:42Z","timestamp":1774576182000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.70790"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/tpj.70790"],"URL":"https:\/\/doi.org\/10.1111\/tpj.70790","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-11-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70790"}}