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However, the molecular mechanisms underlying\n                    <jats:italic>Pt<\/jats:italic>\n                    effector\u2010mediated wheat susceptibility remain largely unexplored. In this study, an effector Pt_20 that is induced expression at the early stage of\n                    <jats:italic>Pt<\/jats:italic>\n                    infection to wheat was identified. Interestingly, the\n                    <jats:italic>Pt_20<\/jats:italic>\n                    gene exhibits two distinct transcriptional isoforms (\n                    <jats:italic>Pt_20<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>636<\/jats:italic>\n                    <\/jats:sub>\n                    ,\n                    <jats:italic>Pt_20<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>807<\/jats:italic>\n                    <\/jats:sub>\n                    ) among\n                    <jats:italic>Pt<\/jats:italic>\n                    races and both are localized in the nucleus, cytoplasm, and plasma membrane. Pt_20\n                    <jats:sub>636<\/jats:sub>\n                    was able to suppress BAX\u2010mediated cell death in\n                    <jats:italic>Nicotiana benthamiana,<\/jats:italic>\n                    but\n                    <jats:italic>Pt_20<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>807<\/jats:italic>\n                    <\/jats:sub>\n                    was not. Importantly, Pt_20\n                    <jats:sub>636<\/jats:sub>\n                    triggers\n                    <jats:italic>Lr16<\/jats:italic>\n                    \u2010dependent cell death, though Pt_20\n                    <jats:sub>807<\/jats:sub>\n                    does not trigger any immune response. We found that TaLhcb4.1 interacts with Pt_20\n                    <jats:sub>636<\/jats:sub>\n                    , and Pt_20\n                    <jats:sub>636<\/jats:sub>\n                    inhibited the function of TaLhcb4.1 involved in wheat resistance to\n                    <jats:italic>Pt<\/jats:italic>\n                    via the ROS pathway. Furthermore, TaLhcb4.1 facilitates Pt_20\n                    <jats:sub>636<\/jats:sub>\n                    \u2010triggered immune responses in TcLr16 by promoting H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    accumulation. Overall, these findings demonstrate the dual role of Pt_20\n                    <jats:sub>636<\/jats:sub>\n                    , it subverts TaLhcb4.1\u2010mediated resistance while eliciting Lr16\u2010recognized immune signaling, highlighting the complex interplay between fungal virulence and host defense during wheat\u2010\n                    <jats:italic>Pt<\/jats:italic>\n                    interactions.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70791","type":"journal-article","created":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T16:05:31Z","timestamp":1773590731000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Puccinia triticina<\/i>\n                    effector Pt_20\n                    <sub>636<\/sub>\n                    triggers\n                    <scp>PCD<\/scp>\n                    in\n                    <scp>TcLr16<\/scp>\n                    wheat promoted by\n                    <scp>TaLhcb4<\/scp>\n                    .1 via the\n                    <scp>ROS<\/scp>\n                    pathway"],"prefix":"10.1111","volume":"125","author":[{"given":"Songsong","family":"Shen","sequence":"first","affiliation":[{"name":"College of Plant Protection, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxin","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linshuo","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyue","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shitao","family":"Yuan","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Agriculture in Northern Mountainous Areas Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8972-5662","authenticated-orcid":false,"given":"Zhongchi","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daqun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihui","family":"Wu","sequence":"additional","affiliation":[{"name":"Wheat Research Institute Tangshan Academy of Agricultural Sciences  Tangshan Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province Hebei Agricultural University  Baoding Hebei 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