{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T16:10:45Z","timestamp":1785946245849,"version":"3.56.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100019180","name":"HORIZON EUROPE European Research Council","doi-asserted-by":"publisher","award":["949618"],"award-info":[{"award-number":["949618"]}],"id":[{"id":"10.13039\/100019180","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["031B0188"],"award-info":[{"award-number":["031B0188"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Meiosis is a key process in sexual reproduction, orchestrated by numerous proteins that differ in their spatiotemporal expression level and dynamics. Employing meiotic gene promoters typically leads to varying levels of transgene expression within meiotic and non\u2010meiotic cells in plants. Hence, their application for protein expression during meiosis is limited. Here, we present a tool for meiosis\u2010specific protein co\u2010expression in\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    . By utilizing ribosome\u2010skipping peptides, we leverage the distinct expression patterns of the two meiotic genes\n                    <jats:italic>ASY1<\/jats:italic>\n                    and\n                    <jats:italic>MTOPVIB<\/jats:italic>\n                    to co\u2010express various proteins through a single polyprotein precursor containing the linker peptides P2a or IntF2a with \u2018self\u2010cleaving\u2019 abilities. During translation of ASY1 or MTOPVIB in prophase I, those linkers of viral origin facilitate the separation of the polyprotein precursor components through a ribosomal \u2018skip\u2019 and their release as individual proteins in a one\u2010to\u2010one ratio. Compared with P2a, IntF2a achieves more efficient cleavage in male meiocytes. We used our system for meiosis\u2010specific co\u2010expression of up to two additional proteins. For example, by applying light sheet fluorescence microscopy, we studied the spatiotemporal dynamics of H2B, CENH3, and alpha\u2010tubulin isoform TUA6 in combination with three different fluorescent proteins during male meiosis. Overall, we achieved coordinated co\u2010synthesis of up to three proteins from a single polyprotein precursor, allowing synchronized co\u2010expression of proteins at the level of either ASY1 or MTOPVIB during meiosis. In summary, the approach provides a versatile and efficient tool for studying or potentially modifying meiotic processes through precise and coordinated protein expression during meiosis or other biotechnological applications requiring coordinated protein co\u2010synthesis in plants.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70811","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T19:11:11Z","timestamp":1774465871000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Meiosis\u2010specific protein co\u2010expression in\n                    <i>Arabidopsis thaliana<\/i>\n                    : a protein delivery tool in meiotic cells"],"prefix":"10.1111","volume":"125","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4752-6822","authenticated-orcid":false,"given":"Ewa W.","family":"Piskorz","sequence":"first","affiliation":[{"name":"Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben  Corrensstrasse 3 06466 Seeland Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0009-3724","authenticated-orcid":false,"given":"Stefan","family":"Steckenborn","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben  Corrensstrasse 3 06466 Seeland Germany"},{"name":"Max Planck Institute for Plant Breeding Research  Carl\u2010von\u2010Linne\u2010Weg 10 D\u201050829 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4910-7311","authenticated-orcid":false,"given":"Maria","family":"Cuacos","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben  Corrensstrasse 3 06466 Seeland Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0189-8428","authenticated-orcid":false,"given":"Stefan","family":"Heckmann","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben  Corrensstrasse 3 06466 Seeland Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00048"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-103-5_9"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)00423-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4074"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7492"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.16.00922"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u2010313x.1998.00343.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.11.9.1623"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12647"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022\u20101317\u201082\u20105\u20101013"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13601"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.6.1349"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477\u2010023\u201001371\u20107"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010794"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1738"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467\u20107652.2004.00113.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13312"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-006-0206-6"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.00829"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005372"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-050213-035923"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.039644"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42834"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460\u20102075.1994.tb06337.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000121079"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.439007"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.124.4.1477"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1508\/cytologia.84.107"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010021\u201002106\u20100"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2022.08.014"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.39.24480"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010425"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.52546"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad5196"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab715"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhac056"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019101625"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12670"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14442"}],"container-title":["The 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