{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:21:07Z","timestamp":1787016067534,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Camptothecin is a valuable monoterpene indole alkaloid (MIA) with antitumor activity, and strictosamide represents the first specialized intermediate in camptothecin biosynthesis. The proposed camptothecin biosynthetic pathway involves several oxidation\u2013reduction reactions, while there have been no reports on the involvement of 2\u2010oxoglutarate\u2010dependent dioxygenases (2OGDs) in\n                    <jats:italic>Ophiorrhiza pumila<\/jats:italic>\n                    . In this study, the genome\u2010wide identification of 2OGDs was performed in\n                    <jats:italic>O. pumila<\/jats:italic>\n                    , and a total of 140\n                    <jats:italic>2OGD<\/jats:italic>\n                    genes were identified. Functional characterization revealed that five Op2OGDs catalyze the dehydrogenation of the C\u20103 and C\u201014 positions of strictosamide to produce dehydrostrictosamide. Unexpectedly, strictosamide was found to undergo a rapid flavin\u2010mediated photocatalytic skeletal rearrangement to form pumiloside. According to the chromosomal localization of functional\n                    <jats:italic>Op2OGD<\/jats:italic>\n                    genes and camptothecin biosynthetic genes, we identified an MIA gene cluster on chromosome 5 of\n                    <jats:italic>O. pumila<\/jats:italic>\n                    , including two 2OGDs identified in this study and four previously characterized genes. These findings support that camptothecin biosynthesis in\n                    <jats:italic>O.\u00a0pumila<\/jats:italic>\n                    proceeds through a biosynthetic network rather than a linear pathway. This study provides important insights into the elucidation of the camptothecin biosynthetic pathway.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70823","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T21:34:37Z","timestamp":1774992877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Enzymatic dehydrogenation and flavin\u2010mediated photocatalytic skeletal rearrangement of strictosamide, a\u00a0key\u00a0intermediate in camptothecin biosynthesis"],"prefix":"10.1111","volume":"126","author":[{"given":"Xinyao","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Medicinal Plant Development Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing 100193 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Medicinal Plant Development Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing 100193 China"},{"name":"Yunnan Key Laboratory of Southern Medicinal Resources, Yunnan Branch Institute of Medicinal Plant Development Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Jinghong 666100 China"},{"name":"Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Engineering Research Center of Chinese Medicine Resource Ministry of Education  Beijing 100193 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