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It can impact specific protein activity via\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation. We found that protein tyrosine phosphatase 1 (PTP1) participates in the response of\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    (\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>thaliana<\/jats:italic>\n                    ) to both high\u2010salt and freezing conditions. NO facilitated PTP1\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation at cysteine (Cys)\u201041, resulting in increased salt tolerance and altered sensitivity to freezing. Additionally, this process reduced the dephosphorylation activity of PTP1 and activated mitogen\u2010activated protein kinase 6 (MPK6) in plants exposed to high\u2010salt and freezing conditions. MPK6 activation increased plant resistance to salt stress but attenuated plant freezing tolerance. Genetic analysis revealed that the effects of PTP1 on salinity and freezing stresses were dependent on MPK6 activity. Collectively, our findings show a new mechanism by which NO negatively influences the enzymatic activity of PTP1 via\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation at Cys\u201041. This process, in turn, influences the levels of phosphorylated MPK6 in\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    , enhancing its ability to tolerate salt loading and to respond to freezing conditions.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70844","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T22:45:52Z","timestamp":1774997152000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>S<\/i>\n                    \u2010nitrosylation of\n                    <scp>PTP1<\/scp>\n                    positively regulates\n                    <scp>MPK6<\/scp>\n                    activity in response to salt or cold stress in\n                    <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1111","volume":"126","author":[{"given":"Xiaoying","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences Hebei Normal University  Shijiazhuang 050024 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaojiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences Hebei Normal University  Shijiazhuang 050024 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Biology and Food Science Hebei Normal University for Nationalities  Chengde 067000 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