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Here, we identify the E3 ubiquitin ligase Plant U\u2010box 26 (VvPUB26) as a pivotal regulator of the grapevine cold signaling pathway. We demonstrated that the VvPUB26\u2010VvPIF4\u2010VvCBF3 module fine\u2010tunes cold tolerance by integrating light and temperature signals. Overexpression of\n                    <jats:italic>VvPUB26<\/jats:italic>\n                    (\n                    <jats:italic>VvPUB26\u2010OE<\/jats:italic>\n                    ) significantly enhances cold tolerance in transgenic plants, whereas RNA interference (\n                    <jats:italic>VvPUB26\u2010RNAi<\/jats:italic>\n                    ) lines exhibit increased cold sensitivity. Biochemical analysis reveals that VvPUB26 modulates the CBF signaling cascade by interacting with several key cold\u2010related proteins, including VvMYB15, VvICEs, and VvPIF4. Specifically, VvPUB26 facilitates the ubiquitination and subsequent degradation of VvPIF4 and VvMYB15, while simultaneously maintaining the stability of VvICEs protein. Although the VvPIF4 protein typically accumulates under cold stress and darkness, VvPUB26\u2010mediated degradation weakens this stability. Furthermore, VvPIF4 directly binds to the\n                    <jats:italic>VvCBF3<\/jats:italic>\n                    promoter to repress its transcription, thereby negatively regulating cold tolerance. Overexpression of\n                    <jats:italic>VvPIF4<\/jats:italic>\n                    (\n                    <jats:italic>VvPIF4\u2010OE<\/jats:italic>\n                    ) phenocopies the cold\u2010sensitive\n                    <jats:italic>VvPUB26\u2010RNAi<\/jats:italic>\n                    lines, confirming its role as a negative regulator. Our findings establish that the VvPUB26\u2010VvPIF4\u2010VvCBF3 module optimizes grapevine cold adaptation through ubiquitination\u2010dependent protein turnover, effectively linking light\u2010temperature crosstalk to the CBF\u2010COR pathway.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70878","type":"journal-article","created":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T05:50:21Z","timestamp":1776577821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>E3<\/scp>\n                    ubiquitin ligase\n                    <scp>VvPUB26<\/scp>\n                    targets\n                    <scp>VvPIF4<\/scp>\n                    for degradation to positively regulate cold resistance in grapevine"],"prefix":"10.1111","volume":"126","author":[{"given":"Ting","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Horticulture Northwest A&amp;F University  Yangling Shaanxi 712100 China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region) Ministry of Agriculture, P.R. China  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Horticulture Northwest A&amp;F University  Yangling Shaanxi 712100 China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region) Ministry of Agriculture, P.R. China  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyin","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Horticulture Northwest A&amp;F University  Yangling Shaanxi 712100 China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region) Ministry of Agriculture, P.R. China  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqi","family":"Lian","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Horticulture Northwest A&amp;F University  Yangling Shaanxi 712100 China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region) Ministry of Agriculture, P.R. China  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqing","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Horticulture Northwest A&amp;F University  Yangling Shaanxi 712100 China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region) Ministry of Agriculture, P.R. China  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congbo","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Horticulture Northwest A&amp;F University  Yangling Shaanxi 712100 China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region) Ministry of Agriculture, P.R. China  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Horticulture Northwest A&amp;F University  Yangling Shaanxi 712100 China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region) Ministry of Agriculture, P.R. China  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University  Yangling Shaanxi 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaohong","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Horticulture Northwest A&amp;F University  Yangling Shaanxi 712100 China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region) Ministry of Agriculture, P.R. 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