{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T20:57:57Z","timestamp":1781816277569,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T00:00:00Z","timestamp":1776470400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100006206","name":"Biological and Environmental Research","doi-asserted-by":"publisher","award":["DE\u2010SC0023160"],"award-info":[{"award-number":["DE\u2010SC0023160"]}],"id":[{"id":"10.13039\/100006206","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006206","name":"Biological and Environmental Research","doi-asserted-by":"publisher","award":["DE\u2010SC0018277"],"award-info":[{"award-number":["DE\u2010SC0018277"]}],"id":[{"id":"10.13039\/100006206","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Setaria viridis<\/jats:italic>\n                    is a diploid C\n                    <jats:sub>4<\/jats:sub>\n                    grass in the Poaceae family, notable for its rapid life cycle of 6\u20138\u2009weeks from sowing to seed\u2014much shorter than the 4\u20135\u2009months required by crops such as\n                    <jats:italic>Zea mays<\/jats:italic>\n                    and\n                    <jats:italic>Sorghum bicolor<\/jats:italic>\n                    . This fast growth makes\n                    <jats:italic>S. viridis<\/jats:italic>\n                    a valuable model for C\n                    <jats:sub>4<\/jats:sub>\n                    crop research. Genetic crosses are essential for studying gene function, but manual crossing is labor\u2010intensive and time\u2010consuming. To address this, we developed a male\u2010sterile line by targeting the\n                    <jats:italic>S. viridis<\/jats:italic>\n                    ortholog of\n                    <jats:italic>Setaria italica NO POLLEN 1<\/jats:italic>\n                    (\n                    <jats:italic>SiNP1<\/jats:italic>\n                    ), which encodes a glucose\u2013methanol\u2013choline oxidoreductase required for pollen exine formation. Using\n                    <jats:italic>Cas9<\/jats:italic>\n                    and\n                    <jats:italic>TREX2<\/jats:italic>\n                    \u2010mediated genome editing, we generated\n                    <jats:italic>SiNP1<\/jats:italic>\n                    knockouts in both the\n                    <jats:italic>S. viridis<\/jats:italic>\n                    ME034V and A10.1 backgrounds that were fully male\u2010sterile. Backcrossing T\n                    <jats:sub>0<\/jats:sub>\n                    male\u2010sterile plants to ME034V wild\u2010type followed by selfing yielded a stable BC\n                    <jats:sub>1<\/jats:sub>\n                    F\n                    <jats:sub>2<\/jats:sub>\n                    line homozygous for a 59\u2009bp deletion in the\n                    <jats:italic>S. viridis NO POLLEN 1<\/jats:italic>\n                    gene, easily genotyped by PCR and maintained by heterozygous siblings. Using this line, we developed a simple and efficient crossing protocol that eliminates the need for emasculation. This method enables a single person to perform up to 100 crosses per day\u2014compared to 15 using traditional methods\u2014and yields 20\u201332\u2009F\n                    <jats:sub>1<\/jats:sub>\n                    hybrid seeds per panicle with 100% genetic purity. We also quantified pollen flow and outcrossing frequencies under greenhouse conditions to develop optimal bagging strategies and prevent unintended pollination. This resource accelerates genetic research in\n                    <jats:italic>S. viridis<\/jats:italic>\n                    , enhancing its utility as a premier C\n                    <jats:sub>4<\/jats:sub>\n                    model for mapping and functional genomics.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70880","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T16:49:22Z","timestamp":1776530962000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of male\u2010sterile lines of\n                    <i>Setaria viridis<\/i>\n                    to accelerate\n                    <scp>\n                      C\n                      <sub>4<\/sub>\n                    <\/scp>\n                    model plant genetics"],"prefix":"10.1111","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4588-7074","authenticated-orcid":false,"given":"Hui","family":"Jiang","sequence":"first","affiliation":[{"name":"Donald Danforth Plant Sciences Center  Saint Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3523-7307","authenticated-orcid":false,"given":"Erik A.","family":"Myers","sequence":"additional","affiliation":[{"name":"Department of Genetics, Cell Biology and Development and Center for Precision Plant Genomics University of Minnesota  St. Paul Minnesota USA"},{"name":"Plant and Microbial Biology Graduate Program University of Minnesota  St. Paul Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britney","family":"Millman","sequence":"additional","affiliation":[{"name":"Donald Danforth Plant Sciences Center  Saint Louis Missouri 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6680-1722","authenticated-orcid":false,"given":"Ivan","family":"Baxter","sequence":"additional","affiliation":[{"name":"Donald Danforth Plant Sciences Center  Saint Louis Missouri 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