{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:14:48Z","timestamp":1778818488153,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170588"],"award-info":[{"award-number":["32170588"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2243200012"],"award-info":[{"award-number":["2243200012"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2025A1515011039"],"award-info":[{"award-number":["2025A1515011039"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    In Arabidopsis, the nucleotidyl transferase UTP: RNA URIDYLYLTRANSFERASE (URT1) has been identified as a key mediator of mRNA uridylation and metabolic regulation. While its biochemical function in RNA modification has been established, the physiological relevance of URT1 across different developmental stages remains poorly understood. This study revealed that URT1 exhibits genetic interactions similar to those of EXORIBONUCLEASE 4 (XRN4) during developmental transitions, particularly at the globular embryo stage and during seedling establishment. Simultaneous dysfunction of URT1 and the SUPER\u2010KILLER (SKI) complex resulted in pronounced embryonic lethality during the globular\u2010to\u2010heart stage transition, with residual mortality persisting through seedling development. Small RNA deep sequencing (sRNA\u2010seq) analyses identified certain members of the HEAT SHOCK PROTEIN 90\/70 (HSP90\/70) families as primary targets of URT1 and SKI2 during embryogenesis. Our findings demonstrate that URT1 cooperates with the SKI complex to suppress the production of RDR6\u2010dependent aberrant small interfering RNAs (siRNAs) from\n                    <jats:italic>HSP90<\/jats:italic>\n                    \/\n                    <jats:italic>70<\/jats:italic>\n                    loci during embryonic development. In addition, transcriptome profiling demonstrated that URT1 selectively targets a distinct subset of XRN4 substrate mRNAs in seedlings, demonstrating a possible role for URT1 in the 5\u2032\u20133\u2032 cytosolic mRNA decay pathway. Notably, through characterization of the prime editor\u2010generated catalytically inactive\n                    <jats:italic>urt1<\/jats:italic>\n                    allele, we confirmed that the catalytic uridylation activity of URT1 is critical for plant development. Collectively, our findings establish URT1 as an important developmental regulator that orchestrates 5\u2032\u20133\u2032 cytosolic mRNA decay through uridylation activity, highlighting its essential role in maintaining RNA homeostasis during embryogenesis and seedling establishment.\n                  <\/jats:p>","DOI":"10.1111\/tpj.70924","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T23:09:22Z","timestamp":1778713762000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The nucleotidyl transferase\n                    <scp>URT1<\/scp>\n                    \u2010directed\n                    <scp>mRNA<\/scp>\n                    surveillance\u00a0is critical for embryo and seedling development in Arabidopsis"],"prefix":"10.1111","volume":"126","author":[{"given":"Jiwei","family":"Chen","sequence":"first","affiliation":[{"name":"Center for Biological Science and Technology, Faculty of Arts and Sciences, Advanced Institute of Natural Sciences Beijing Normal University  Zhuhai 519087 China"},{"name":"The Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education, Department of Biology, College of Life Sciences Beijing Normal University  Beijing 100875 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozhen","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Biological Science and Technology, Faculty of Arts and Sciences, Advanced Institute of Natural Sciences Beijing Normal University  Zhuhai 519087 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwen","family":"Kong","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography Shenzhen University  Shenzhen 518060 China"},{"name":"Key Laboratory of Plant Molecular Physiology, Institute of Botany Chinese Academy of Sciences  Beijing 100093 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumei","family":"Ren","sequence":"additional","affiliation":[{"name":"Center for Biological Science and Technology, Faculty of Arts and Sciences, Advanced Institute of Natural Sciences Beijing Normal University  Zhuhai 519087 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kangwen","family":"Lao","sequence":"additional","affiliation":[{"name":"Center for Biological Science and Technology, Faculty of Arts and Sciences, Advanced Institute of Natural Sciences Beijing Normal University  Zhuhai 519087 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetics and Development of Complex Phenotypes, Zhangjiang mRNA Innovation and Translation Center, Institute of Plant Biology, School of Life Sciences Fudan University  Shanghai 200438 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yichen","family":"Wu","sequence":"additional","affiliation":[{"name":"Center for Biological Science and Technology, Faculty of Arts and Sciences, Advanced Institute of Natural Sciences Beijing Normal University  Zhuhai 519087 China"},{"name":"Guangdong Institute of Intelligence Science and Technology  Hengqin Zhuhai 519031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangdong Institute of Intelligence Science and Technology  Hengqin Zhuhai 519031 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianxin","family":"Dong","sequence":"additional","affiliation":[{"name":"Instrumentation and Service Center for Science and Technology Beijing Normal University  Zhuhai 519087 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