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Understanding of circadian coordination of CAM remains limited to rhythmic post\u2010translational regulation of phosphoenolpyruvate carboxylase (PPC) by a specific clock\u2010controlled protein kinase, PPCK. Here, candidate promoter regions (~3000\u2009bp) of CAM\u2010associated genes from\n                    <jats:italic>Kalancho\u00eb laxiflora<\/jats:italic>\n                    Baker were coupled to a firefly luciferase reporter and stable transgenic lines of both\n                    <jats:italic>K. laxiflora<\/jats:italic>\n                    and C\n                    <jats:sub>3<\/jats:sub>\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    were generated. In\n                    <jats:italic>K. laxiflora,<\/jats:italic>\n                    the CAM\u2010associated\n                    <jats:italic>GLUCOSE 6\u2010PHOSPHATE\/PHOSPHATE TRANSLOCATOR2<\/jats:italic>\n                    promoter (\n                    <jats:italic>KlGPT2p<\/jats:italic>\n                    ) generated robust circadian rhythms of luciferase luminescence in constant conditions, with peak activity in leaf pair 3, where CAM\u2010associated nocturnal CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation initiated during leaf development.\n                    <jats:italic>KlGPT2p::LUC+<\/jats:italic>\n                    did not drive rhythms of luminescence in Arabidopsis and the\n                    <jats:italic>KlPPCK1<\/jats:italic>\n                    promoter produced no LUC+ signal in either species. Furthermore, the\n                    <jats:italic>CHLOROPHYLL A\/B BINDING PROTEIN2<\/jats:italic>\n                    promoter (\n                    <jats:italic>KlCAB2p<\/jats:italic>\n                    ), a clock\u2010controlled promoter that drives a gene involved in light\u2010reactions of photosynthesis, drove robust rhythms in both\n                    <jats:italic>K.\u00a0laxiflora<\/jats:italic>\n                    and Arabidopsis.\n                    <jats:italic>KlCAB2p<\/jats:italic>\n                    circadian period changed during leaf development in\n                    <jats:italic>K. laxiflora,<\/jats:italic>\n                    and peak activity shifted from dusk in CAM leaves of\n                    <jats:italic>K. laxiflora<\/jats:italic>\n                    to dawn in Arabidopsis, highlighting differences in the timing of outputs from the core clock between species. These findings establish a robust\n                    <jats:italic>PROMOTER::LUC+<\/jats:italic>\n                    reporter system in a CAM plant, highlight divergent timing driving clock\u2010controlled promoters between species, and period lengthening with leaf age in\n                    <jats:italic>Kalancho\u00eb<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/tpj.70937","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:05:56Z","timestamp":1780639556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A\n                    <i>PROMOTER::LUCIFERASE<\/i>\n                    reporter system reveals key elements of the circadian regulation of\n                    <scp>Crassulacean<\/scp>\n                    acid metabolism (\n                    <scp>CAM<\/scp>\n                    ) in\n                    <i>Kalancho\u00eb laxiflora<\/i>\n                    Baker"],"prefix":"10.1111","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9770-8444","authenticated-orcid":false,"given":"Jessica H.","family":"Pritchard","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology Institute of Systems, Molecular and Integrative Biology, University of Liverpool  Crown Street Liverpool L69 7ZB UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0922-4284","authenticated-orcid":false,"given":"Jade L.","family":"Waller","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Systems Biology Institute of Systems, Molecular and Integrative Biology, University of Liverpool  Crown Street Liverpool L69 7ZB UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0709-1190","authenticated-orcid":false,"given":"Peter J. 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