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Sibiricose A5 and A6, two phenylpropanoid sucrose esters (PSEs) with potent antidepressant and neuroprotective activities, are commonly isolated from the roots of the medicinal plant\n                    <jats:italic>Polygala tenuifolia<\/jats:italic>\n                    . Here, we confirmed the root\u2010specific accumulation of these PSEs and assembled a high\u2010quality, chromosome\u2010scale genome for\n                    <jats:italic>P. tenuifolia<\/jats:italic>\n                    (678.38\u2009Mb), achieving a BUSCO completeness of 99.30%. Through integrated phylogenetic and cross\u2010tissue gene\u2010metabolite correlation analyses, we selected root\u2010specific BAHD acyltransferases candidates. Whole\u2010cell feeding assays and\n                    <jats:italic>in vitro<\/jats:italic>\n                    enzyme assays subsequently identified Sucrose Hydroxycinnamoyl Transferases PtSHT1 and PtSHT2 as the enzymes responsible for the biosynthesis of both sibiricose A5 and A6. We further discovered a BAHD acyltransferase conserved within a syntenic genomic segment across angiosperms, representing the ancestral lineage of the sucrose ferulic acid transferases from\n                    <jats:italic>Z. mays<\/jats:italic>\n                    (ZmSFTs). However, these conserved enzymes lacked sibiricose A5\/A6 synthetic activity, and the\n                    <jats:italic>P. tenuifolia<\/jats:italic>\n                    homolog was not expressed in roots. Phylogenetic analysis revealed that PtSHTs and ZmSFTs form two distinct monophyletic clades: PtSHTs belonging to acyltransferases clade 3, a eudicot\u2010specific lineage, whereas ZmSFTs belong to clade 6. Furthermore, combinatorial expression of PtSHTs with auxiliary acyltransferases, selected based on syntenic context and correlation analysis, yielded several novel PSEs. These findings advance our understanding of PSE biosynthesis and the independent evolutionary recruitment of SHT enzymes with sibiricose A5 and A6 synthetic activity, while also facilitating the targeted production of specific PSEs for pharmaceutical applications.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71000","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T22:04:43Z","timestamp":1782770683000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Independent recruitment of enzymes for phenylpropanoid sucrose ester biosynthesis in\n                    <i>Polygala tenuifolia<\/i>"],"prefix":"10.1111","volume":"127","author":[{"given":"Renyu","family":"Deng","sequence":"first","affiliation":[{"name":"College of Plant Science and Technology Huazhong Agricultural University  Wuhan 430070 China"},{"name":"Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen 518124 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen 518124 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongling","family":"Tian","sequence":"additional","affiliation":[{"name":"Chinese Materia Medica China Agriculture Research System (CARS\u201021)  Fenyang Shanxi 032200 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Jander","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute  533 Tower Road Ithaca New York 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3643-7875","authenticated-orcid":false,"given":"Shaoqun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen 518124 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences  Shenzhen 518124 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","unstructured":"Bai J. Li J. Zhang Y. Chang H. Zhang N. Liu Y.et\u00a0al. 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Somani A. Liu J.C.\u2010T. Karunadasa S.S. Xu S.\u2010L. Fordyce P.M.et\u00a0al. 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