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In doing so, it is assumed that changing\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>a<\/jats:sub>\n                    essentially alters the carboxylation\u2010to\u2010oxygenation ratio, with negligible effects on other metabolic pathways. There is now substantial evidence that this assumption is incorrect, with strong modifications in day respiratory metabolism, N and S assimilation and cytosolic translational activity. In principle, these modifications should reconfigure protein content and composition which might in turn affect photosynthetic activity. To address this point, we carried out gas exchange experiments on sunflower leaves under varying CO\n                    <jats:sub>2<\/jats:sub>\n                    , either using a portable system or a special leaf chamber designed for\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010labelling. Non\u2010targeted analysis of metabolites, proteins and isotopic features was performed to look at metabolites, protein synthesis rate and proteins affected by CO\n                    <jats:sub>2<\/jats:sub>\n                    mole fraction within 1 to 3\u2009h. Proteogenic amino acids and amino acid derivatives were amongst the most affected metabolites. Protein synthesis rate increased with\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>a<\/jats:sub>\n                    , except at very high CO\n                    <jats:sub>2<\/jats:sub>\n                    . Accordingly, proteins affected by\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>a<\/jats:sub>\n                    included proteins involved in translation (ribosomal proteins, initiation factors) and proteases, showing a change in protein turn\u2010over. Also,\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>a<\/jats:sub>\n                    impacted some proteins of the photosynthetic machinery. Taken as a whole, our results demonstrate that CO\n                    <jats:sub>2<\/jats:sub>\n                    mole fraction has broad short\u2010term effects on leaf protein\u2010related metabolism. In principle, this effect should have consequences on several aspects of photosynthesis, such as day respiration or photosynthetic capacity itself.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71008","type":"journal-article","created":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T19:04:06Z","timestamp":1783191846000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid\n                    <scp>\n                      CO\n                      <sub>2<\/sub>\n                    <\/scp>\n                    effects on protein\u2010related metabolism during photosynthetic gas exchange"],"prefix":"10.1111","volume":"127","author":[{"given":"Corentin","family":"Dourmap","sequence":"first","affiliation":[{"name":"Univ Angers, Institut Agro, INRAE, IRHS, SFR QUASAV  F\u201049000 Angers 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