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The eukaryotic green alga\n                    <jats:italic>Chlamydomonas<\/jats:italic>\n                    (\n                    <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>\n                    ) maintains efficient phototrophic growth in ambient or low CO\n                    <jats:sub>2<\/jats:sub>\n                    environments by establishing a carbon concentrating mechanism (CCM). We show that\n                    <jats:italic>Chlamydomonas<\/jats:italic>\n                    raises its cellular Zn quota to accommodate higher Zn demand under low CO\n                    <jats:sub>2<\/jats:sub>\n                    conditions, an adjustment that is dependent on CIA5, a major regulator of the CCM. We demonstrate that\n                    <jats:italic>CIA5<\/jats:italic>\n                    is constitutively expressed regardless of Zn supply, but its encoded protein undergoes proteasomal degradation under Zn deficiency, providing a checkpoint that prevents CCM induction when Zn cofactors are unavailable. We identified a COBW domain\u2010containing GTPase, ZNG3, as a constitutive interacting partner of CIA5. As CIA5, ZNG3 does not accumulate in low Zn and is degraded by the proteasome. Unlike\n                    <jats:italic>cia5<\/jats:italic>\n                    mutants,\n                    <jats:italic>zng3<\/jats:italic>\n                    mutants grow like wild\u2010type without CO\n                    <jats:sub>2<\/jats:sub>\n                    supplementation but exhibit a growth defect when grown in the presence of high CO\n                    <jats:sub>2<\/jats:sub>\n                    or acetate. Transcriptome sequencing revealed that expression of genes encoding central components of the CCM is mostly unchanged in\n                    <jats:italic>zng3<\/jats:italic>\n                    mutants, while a subset of genes induced in Zn deficiency, including\n                    <jats:italic>ZRT2<\/jats:italic>\n                    encoding for a Zn transporter, is increasingly expressed. Under low CO\n                    <jats:sub>2<\/jats:sub>\n                    conditions,\n                    <jats:italic>cia5 zng3<\/jats:italic>\n                    double mutants exhibit a more severe phenotype than\n                    <jats:italic>cia5<\/jats:italic>\n                    single mutants. This result points to a condition\u2010dependent genetic interaction between\n                    <jats:italic>CIA5<\/jats:italic>\n                    and\n                    <jats:italic>ZNG3<\/jats:italic>\n                    , suggesting both proteins provide distinct but partially overlapping contributions to fitness depending on carbon availability.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71035","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:03:36Z","timestamp":1784073816000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CIA5<\/scp>\n                    and its interacting metal\u2010binding\n                    <scp>GTPase ZNG3<\/scp>\n                    are degraded by the proteasome in Zn deficiency"],"prefix":"10.1111","volume":"127","author":[{"given":"George","family":"Kusi\u2010Appiah","sequence":"first","affiliation":[{"name":"Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan 48824 USA"},{"name":"Department of Biochemistry and Molecular Biology Michigan State University  East Lansing Michigan 48824 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schmollinger","sequence":"additional","affiliation":[{"name":"Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan 48824 USA"},{"name":"Department of Biochemistry and Molecular Biology Michigan State University  East Lansing Michigan 48824 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Mamo","sequence":"additional","affiliation":[{"name":"Department of Physiology Michigan State University  East Lansing 48824 Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah C.","family":"Stainbrook","sequence":"additional","affiliation":[{"name":"Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan 48824 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8732-5059","authenticated-orcid":false,"given":"Thomas V.","family":"O'Halloran","sequence":"additional","affiliation":[{"name":"Department of Chemistry Michigan State University  East Lansing Michigan 48824 USA"},{"name":"Department of Microbiology, Genetics and Immunology Michigan State University  East Lansing Michigan 48824 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3420-1332","authenticated-orcid":false,"given":"Daniela","family":"Strenkert","sequence":"additional","affiliation":[{"name":"Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan 48824 USA"},{"name":"Department of Plant Biology Michigan State University  East Lansing Michigan 48824 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