{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T12:04:45Z","timestamp":1785326685235,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32500247"],"award-info":[{"award-number":["32500247"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2024MC093"],"award-info":[{"award-number":["ZR2024MC093"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Root hairs are essential for nutrient and water uptake and environmental interactions. RSL4 is a key transcription factor regulating their growth, but how hormones regulate RSL4 post\u2010translationally remains unclear. Here, we show that root hair\u2010enriched CPK4\/5\/6\/11 directly phosphorylate RSL4 at Ser94\/95\/111\/131. Phosphodeficient RSL4\n                    <jats:sup>S94\/95\/111\/131A<\/jats:sup>\n                    shows reduced transcriptional activity and fails to rescue the\n                    <jats:italic>rsl4<\/jats:italic>\n                    mutant, whereas phosphomimetic RSL4\n                    <jats:sup>S94\/95\/111\/131D<\/jats:sup>\n                    enhances activity and promotes root hair elongation. Loss of function of CPKs results in slower root hair growth compared with wild\u2010type, and leads to shorter root hairs under normal conditions and in response to auxin and ethylene. Notably, promoters of\n                    <jats:italic>CPK4<\/jats:italic>\n                    and\n                    <jats:italic>CPK11<\/jats:italic>\n                    contain binding motifs for RSL4, ethylene\u2010responsive EIN3, and auxin\u2010responsive ARF5. RT\u2010qPCR, LUC reporter assays, and ChIP assays confirm that these transcription factors directly regulate\n                    <jats:italic>CPK4<\/jats:italic>\n                    and\n                    <jats:italic>CPK11<\/jats:italic>\n                    expression. Our findings reveal that RSL4 is post\u2010translationally activated by CPK\u2010mediated phosphorylation, while\n                    <jats:italic>CPK4\/11<\/jats:italic>\n                    are transcriptionally induced by RSL4. Both are further modulated by ethylene and auxin signaling via EIN3 and ARFs, forming a positive feedback loop that integrates transcriptional and phosphorylation events downstream of hormonal signals to drive root hair growth. This work uncovers a hierarchical regulatory network underlying root hair development.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71049","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T04:51:33Z","timestamp":1784782293000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A\n                    <scp>RSL4<\/scp>\n                    \u2010\n                    <scp>CPKs<\/scp>\n                    feedback loop integrates auxin and ethylene signals to regulate root hair growth in Arabidopsis"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7322-2658","authenticated-orcid":false,"given":"Lu\u2010Shen","family":"Li","sequence":"first","affiliation":[{"name":"College of Agriculture and Biology Liaocheng University  Liaocheng 252000 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