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In this study, we have functionally characterized the structured motif\n                    <jats:italic>45ABC<\/jats:italic>\n                    , which is located in the pre\u2010mRNAs of RNA\u2010binding protein (RBP) 45 genes in many plant species. Our data revealed that this element mediates a negative auto\u2010 and cross\u2010regulatory feedback loop via AS of the three\n                    <jats:italic>45ABC<\/jats:italic>\n                    \u2010containing RBP45 genes in\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    . We identified a G\u2010rich stretch within the first stem as a potential RBP45 binding site and observed increased RBP45\u2010dependent AS upon structural weakening of this pairing element. The second stem includes the alternative 5\u2032 splice site being activated in the presence of RBP45. Based on the known interaction between RBP45 homologs and U1 snRNP components required for 5\u2032 splice site recognition, we propose that RBP45 recruitment to stem I of\n                    <jats:italic>45ABC<\/jats:italic>\n                    may induce usage of the alternative 5\u2032 splice site in stem II. The resulting splicing variant is unproductive, thereby diminishing RBP45 expression. Analysing the splicing\u2010regulatory impact of the three\n                    <jats:italic>At<\/jats:italic>\n                    \u2010RBP45 genes in auto\u2010 and cross\u2010regulation and a transcriptome\u2010wide manner revealed unequal genetic redundancy with a major role of\n                    <jats:italic>RBP45B<\/jats:italic>\n                    . Furthermore, phenotypical analysis of single\u2010 and higher\u2010order\n                    <jats:italic>rbp45<\/jats:italic>\n                    mutants pointed at these genes' functions in controlling primary root growth and flowering time. Taken together, we demonstrated that both sequence and structural features of\n                    <jats:italic>45ABC<\/jats:italic>\n                    are critical for proper splicing control, balancing RBP45 expression and functions in plants via a conserved mRNA motif.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71060","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T22:11:57Z","timestamp":1787091117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The structured\n                    <scp>mRNA<\/scp>\n                    element\n                    <i>45ABC<\/i>\n                    mediates auto\u2010 and cross\u2010regulation of\n                    <scp>RBP45<\/scp>\n                    genes via alternative splicing"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1445-4781","authenticated-orcid":false,"given":"Maren","family":"Reinhardt","sequence":"first","affiliation":[{"name":"Institute for Molecular Physiology (imP), University of Mainz  Hanns\u2010Dieter\u2010H\u00fcsch\u2010Weg 17 55128 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1022-7862","authenticated-orcid":false,"given":"Rica","family":"Burgardt","sequence":"additional","affiliation":[{"name":"Institute for Molecular Physiology (imP), University of Mainz  Hanns\u2010Dieter\u2010H\u00fcsch\u2010Weg 17 55128 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5417-495X","authenticated-orcid":false,"given":"Christoph","family":"Engel","sequence":"additional","affiliation":[{"name":"Institute for Molecular Physiology (imP), University of Mainz  Hanns\u2010Dieter\u2010H\u00fcsch\u2010Weg 17 55128 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7014-8224","authenticated-orcid":false,"given":"Maximilian","family":"Sack","sequence":"additional","affiliation":[{"name":"Bioinformatics Group, Department of Computer Science and Interdisciplinary Centre for Bioinformatics Leipzig University  H\u00e4rtelstra\u00dfe 16\u201018 04107 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6681-3624","authenticated-orcid":false,"given":"Zasha","family":"Weinberg","sequence":"additional","affiliation":[{"name":"Bioinformatics Group, Department of Computer Science and Interdisciplinary Centre for Bioinformatics Leipzig University  H\u00e4rtelstra\u00dfe 16\u201018 04107 Leipzig 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