{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:37:22Z","timestamp":1786725442704,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF24OC0095408"],"award-info":[{"award-number":["NNF24OC0095408"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008398","name":"Villum Fonden","doi-asserted-by":"publisher","award":["VIL76900"],"award-info":[{"award-number":["VIL76900"]}],"id":[{"id":"10.13039\/100008398","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Nannochloropsis<\/jats:italic>\n                    and\n                    <jats:italic>Microchloropsis<\/jats:italic>\n                    are important as eustigmatophyte model organisms and the production of high\u2010value lipids. Efficient selectable markers are indispensable for genetic engineering to study and improve production yield of these species. Here, we establish sulfadiazine resistance mediated by the bacterial\n                    <jats:italic>sul1<\/jats:italic>\n                    gene as an efficient and robust selection system. Growth assays identified 300\u2009\u03bcg\u00b7mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    sulfadiazine as an effective selection concentration. Mitochondrial targeting of Sul results in transformation efficiencies and false\u2010positive rates superior to commonly used markers aphVII and Sh ble. Sulfadiazine\u2010resistant transformants exhibit stable growth under selection and no cross\u2010resistance to hygromycin B, Zeocin, blasticidin S, G418, or nourseothricin. Expression of\n                    <jats:italic>Chromochloris zofingiensis \u03b2<\/jats:italic>\n                    \u2010carotene ketolase using the Sul selectable marker in\n                    <jats:italic>Nannochloropsis oceanica<\/jats:italic>\n                    and\n                    <jats:italic>Microchloropsis gaditana<\/jats:italic>\n                    resulted in accumulation of the ketocarotenoids canthaxanthin and adonirubin, producing a distinct brown phenotype. Mitochondrial\u2010targeted Sul thus provides a robust, high\u2010efficiency selectable marker for transformation and genetic engineering in\n                    <jats:italic>Nannochloropsis<\/jats:italic>\n                    and\n                    <jats:italic>Microchloropsis<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/tpj.71063","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T20:25:42Z","timestamp":1785529542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sulfadiazine selection enables high\u2010efficiency transformation in\n                    <i>Nannochloropsis<\/i>\n                    and\n                    <i>Microchloropsis<\/i>"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6813-3809","authenticated-orcid":false,"given":"Jannis","family":"Straube","sequence":"first","affiliation":[{"name":"Plant Biochemistry, Department of Plant and Environmental Sciences University of Copenhagen  Frederiksberg C 1871 Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7593-1000","authenticated-orcid":false,"given":"Christopher Robin","family":"Wood","sequence":"additional","affiliation":[{"name":"Plant Biochemistry, Department of Plant and Environmental Sciences University of Copenhagen  Frederiksberg C 1871 Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8201-4082","authenticated-orcid":false,"given":"Jonna","family":"Pohjankukka","sequence":"additional","affiliation":[{"name":"Plant Biochemistry, Department of Plant and Environmental Sciences University of Copenhagen  Frederiksberg C 1871 Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6597-3431","authenticated-orcid":false,"given":"Johan","family":"Andersen\u2010Ranberg","sequence":"additional","affiliation":[{"name":"Plant Biochemistry, Department of Plant and Environmental Sciences University of Copenhagen  Frederiksberg C 1871 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