{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T17:07:11Z","timestamp":1788973631560,"version":"build-2803163510"},"reference-count":82,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T00:00:00Z","timestamp":1787356800000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100020539","name":"Tennessee Soybean Promotion Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020539","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009260","name":"Institute of Agriculture, University of Tennessee","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009260","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Plant\u2010parasitic nematodes deploy secreted effector proteins that reprogram host cellular processes to establish parasitism. Here, we characterized Mi1D08B, a putative dorsal\u2010gland effector from the root\u2010knot nematode\n                    <jats:italic>Meloidogyne incognita<\/jats:italic>\n                    and defined its role in subverting soybean jasmonate\u2010mediated immunity. Mi1D08B is conserved across several\n                    <jats:italic>Meloidogyne<\/jats:italic>\n                    species and localizes to the plant nucleus and cytoplasm. Overexpression of\n                    <jats:italic>Mi1D08B<\/jats:italic>\n                    significantly increased galling and egg production, demonstrating a strong virulence role. A gall\u2010specific yeast two\u2010hybrid screen identified the NINJA\u2010family co\u2010repressor mc410 as a host target of Mi1D08B. Consistent with the functional relevance of this interaction,\n                    <jats:italic>mc410<\/jats:italic>\n                    promoter activity was detected in galls and giant cells throughout nematode infection. Genetic manipulation of\n                    <jats:italic>mc410<\/jats:italic>\n                    phenocopied Mi1D08B activity as\n                    <jats:italic>mc410<\/jats:italic>\n                    overexpression enhanced susceptibility, whereas\n                    <jats:italic>mc410<\/jats:italic>\n                    silencing reduced nematode infection. Hormone profiling revealed that\n                    <jats:italic>Mi1D08B<\/jats:italic>\n                    overexpression elevated 12\u2010oxo\u2010phytodienoic acid (OPDA) but reduced jasmonic acid (JA) and JA\u2010Ile, consistent with a bottleneck at the peroxisomal OPDA\u2010to\u2010JA conversion. Furthermore, several metabolites associated with this conversion were reduced, indicating that Mi1D08B perturbs metabolic flux through the peroxisomal phase of jasmonate biosynthesis. Gene expression analyses supported this biochemical signature, with upregulation of plastidial OPDA\u2010biosynthetic genes and downregulation of peroxisomal OPDA\u2010reductases, JA\u2010conjugating enzymes, and JA\u2010responsive markers. Together, our data support a model in which Mi1D08B effector interacts with a NINJA co\u2010repressor to suppress JA biosynthesis and signaling, thereby coupling nuclear transcriptional repression to altered metabolism. This mechanism deepens our understanding of nematode manipulation of host hormone networks and highlights the Mi1D08B\u2013mc410 interface and OPDA conversion as promising targets for engineering nematode resistance.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71095","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T09:38:52Z","timestamp":1787391532000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The root\u2010knot nematode effector\n                    <scp>Mi1D08B<\/scp>\n                    targets a\n                    <scp>NINJA<\/scp>\n                    \u2010family co\u2010repressor to suppress jasmonate production and promote infection in soybean"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1900-154X","authenticated-orcid":false,"given":"Peitong","family":"Li","sequence":"first","affiliation":[{"name":"Department of Plant Sciences University of Tennessee  Knoxville Tennessee 37996 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sobhan Bahrami","family":"Zadegan","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee  Knoxville Tennessee 37996 USA"},{"name":"UT\u2010ORNL Graduate School of Genome Science and Technology, University of Tennessee  Knoxville Tennessee 37996 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2040-3806","authenticated-orcid":false,"given":"Nicole","family":"Coffey","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee  Knoxville Tennessee 37996 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandiny","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee  Knoxville Tennessee 37996 USA"},{"name":"UT\u2010ORNL Graduate School of Genome Science and Technology, University of Tennessee  Knoxville Tennessee 37996 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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