{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:07:23Z","timestamp":1789729643276,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32500247"],"award-info":[{"award-number":["32500247"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2024MC093"],"award-info":[{"award-number":["ZR2024MC093"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Calmodulin\u2010binding protein 60b (CBP60b) is a pivotal transcription factor in the transcriptional reprogramming of immune responses. Although its downstream regulatory genes and associated genetic pathways have been reported, its post\u2010translational regulation and modifications remain unexplored. Here, we report that calcium\u2010dependent protein kinases CPK4\/5\/6\/11 physically interact with CBP60b and phosphorylate its serine 568 residue, thereby enhancing its transcriptional activity. The phosphodeficient variant CBP60b\n                    <jats:sup>S568A<\/jats:sup>\n                    compromises immune responses, whereas the phosphomimetic variant CBP60b\n                    <jats:sup>S568D<\/jats:sup>\n                    enhances resistance against pathogens, demonstrating that ser568 is essential for the function of CBP60b. Moreover, we observed that the expression of\n                    <jats:italic>CPK4\/5\/6\/11<\/jats:italic>\n                    is induced by pathogens, while this pathogen\u2010induced expression of\n                    <jats:italic>CPKs<\/jats:italic>\n                    is reduced in the\n                    <jats:italic>cbp60b<\/jats:italic>\n                    mutant. Through experiments including dual luciferase (LUC) reporter assays and chromatin immunoprecipitation (ChIP) assays, we further demonstrated that\n                    <jats:italic>CPK4<\/jats:italic>\n                    ,\n                    <jats:italic>CPK5<\/jats:italic>\n                    , and\n                    <jats:italic>CPK11<\/jats:italic>\n                    are direct transcriptional targets of CBP60b. In conclusion, our results demonstrate for the first time that CBP60b is post\u2010translationally regulated by CPKs via phosphorylation, while the pathogen\u2010induced expression of\n                    <jats:italic>CPKs<\/jats:italic>\n                    is dependent on CBP60b. This mutual regulation establishes a positive feedback loop, which enables plants to mount a robust immune response against pathogens.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71100","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T21:30:01Z","timestamp":1788903001000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>CPKs<\/scp>\n                    \u2010\n                    <scp>CBP60b<\/scp>\n                    positive feedback loop regulates immunity in Arabidopsis"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7322-2658","authenticated-orcid":false,"given":"Lu\u2010Shen","family":"Li","sequence":"first","affiliation":[{"name":"College of Agriculture and Biology Liaocheng University  Liaocheng 252000 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