{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:39:58Z","timestamp":1792618798139,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,9]]},"abstract":"SUMMARY<\/jats:title>\n \n Abiotic stress resistance in plants is often associated with growth retardation. To address this limitation, stress\u2010inducible promoters can be used to generate plants that exhibit both rapid growth and high stress resistance.\n RD29A<\/jats:italic>\n (Response to Desiccation 29A) is a widely used stress\u2010inducible promoter in\n Arabidopsis thaliana<\/jats:italic>\n (\n AtRD29A<\/jats:italic>\n ) that can be activated by multiple factors including NaCl, ABA, and low temperature. Specifically, under cold stress, it is induced through the response to C\u2010repeat\/DREB binding factors (\n CBFs<\/jats:italic>\n ). In this study, we identified five\n CBF3<\/jats:italic>\n homologous genes\u2014\n BpCBF3e<\/jats:italic>\n ,\n BpCBF3b<\/jats:italic>\n ,\n BpCBF3d<\/jats:italic>\n ,\n BpCBF3c<\/jats:italic>\n , and\n BpCBF3a\u2014<\/jats:italic>\n in birch (\n Betula platyphylla<\/jats:italic>\n ), but did not detect an\n RD29A<\/jats:italic>\n homolog. We proposed\n BpCBF3d<\/jats:italic>\n to be the functional\n CBF<\/jats:italic>\n because its expression is strongly regulated by circadian rhythm and cold stress, and it activates downstream cold response marker genes. To confirm this hypothesis, we performed Y1H assays and generated\n BpCBF3d<\/jats:italic>\n overexpression lines driven by two different promoters\u2014\n p35S<\/jats:italic>\n and\n pRD29A<\/jats:italic>\n . Notably, we found that\n BpCBF3d<\/jats:italic>\n did not bind to the\n AtRD29A<\/jats:italic>\n promoter, whereas\n BpCBF3e<\/jats:italic>\n ,\n BpCBF3b<\/jats:italic>\n ,\n BpCBF3c<\/jats:italic>\n , and\n BpCBF3a<\/jats:italic>\n bound strongly, resulting in constitutive expression of heterologous\n AtRD29A<\/jats:italic>\n in birch. This study provides new molecular tools for breeding forest trees with enhanced resilience to environmental change.\n <\/jats:p>","DOI":"10.1111\/tpj.71121","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:32:31Z","timestamp":1790400751000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Constitutive\n AtRD29A<\/i>\n expression reveals divergent\n BpCBF3<\/i>\n regulatory mechanisms in birch"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8297-7269","authenticated-orcid":false,"given":"Mengyan","family":"Ge","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding College of Forestry, Northeast Forestry University Harbin 150040 China"},{"name":"The Center for Basic Forestry Research College of Forestry, Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding College of Forestry, Northeast Forestry University Harbin 150040 China"},{"name":"The Center for Basic Forestry Research College of Forestry, Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding College of Forestry, Northeast Forestry University Harbin 150040 China"},{"name":"The Center for Basic Forestry Research College of Forestry, Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guifeng","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding College of Forestry, Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2859-3066","authenticated-orcid":false,"given":"Zhimin","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding College of Forestry, Northeast Forestry University Harbin 150040 China"},{"name":"The Center for Basic Forestry Research College of Forestry, Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-006-0204-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005787909506"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-007-0360-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2015.139"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-012-1740-9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.70031"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2007.11.002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-013-9723-6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msae089"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1103741108"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017603"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pci230"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/7036"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2022.01.008"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err210"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-020-01710-8"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559-020-1217-3"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsr040"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2011.08.004"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13357"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.047258"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2013.05.002"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3040.1998.00306.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.6299"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2025.101258"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41597-023-02307-8"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(07)00335-1"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abi8455"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erh045"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2004.12.007"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/treephys\/26.5.575"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310573"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.00533"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1522840113"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.029"}],"container-title":["The 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