{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:49:56Z","timestamp":1792147796346,"version":"4.4.0"},"reference-count":63,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372067"],"award-info":[{"award-number":["32372067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Drought severely limits rapeseed productivity, but the role of NAD kinase in this process remains unclear. Here, we identify BnaA07.NADK3 as a drought\u2010 and ABA\u2010inducible peroxisomal NADK in\n                    <jats:italic>Brassica napus<\/jats:italic>\n                    . Overexpression of BnaA07.NADK3 enhanced drought tolerance, whereas CRISPR\/Cas9\u2010edited lines showed reduced drought tolerance. Mechanistically, overexpression of\n                    <jats:italic>BnaA07.NADK3<\/jats:italic>\n                    elevates NADK activity and NADPH accumulation, thereby promoting the scavenging of drought\u2010induced reactive oxygen species (ROS). Transcriptome profiling of\n                    <jats:italic>BnaA07.NADK3<\/jats:italic>\n                    \u2010overexpressing plants revealed significant enrichment of genes involved in ROS detoxification and root development. Both\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    assays demonstrate that BnaA07.NADK3 physically interacts with the peroxisomal import receptor BnaA03.PEX5 (Peroxin 5), suggesting that BnaA07.NADK3 is associated with the peroxisomal protein import machinery required for maintaining ROS homeostasis under stress. In addition, BnaC08.WHY1 directly binds a conserved TTTTTGAC element in the\n                    <jats:italic>BnaA07.NADK3<\/jats:italic>\n                    promoter and activates its transcription. In summary, our findings uncover a previously unrecognized WHY1\u2013NADK3\u2013PEX5 regulatory module that integrates plastid retrograde signaling, peroxisomal redox metabolism, and drought adaptation in\n                    <jats:italic>Brassica napus<\/jats:italic>\n                    , providing promising targets for molecular breeding of drought\u2010tolerant rapeseed cultivars.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71124","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:13:57Z","timestamp":1790835237000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A WHY1\u2013NADK3\u2013PEX5 module confers drought tolerance by maintaining peroxisomal ROS homeostasis in\n                    <i>Brassica napus<\/i>"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7711-0541","authenticated-orcid":false,"given":"Jiaqi","family":"Yan","sequence":"first","affiliation":[{"name":"College of Agronomy Northwest A&amp;F University  Yangling China"},{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture and Rural Affairs  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Qu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture and Rural Affairs  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture and Rural Affairs  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ze","family":"Tian","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture and Rural Affairs  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lieqiong","family":"Kuang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture and Rural Affairs  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Mu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture and Rural Affairs  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanzhong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture and Rural Affairs  Wuhan China"},{"name":"Hubei Hongshan Laboratory  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4219-7879","authenticated-orcid":false,"given":"Xiaoling","family":"Dun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences Ministry of Agriculture and Rural Affairs  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koac344"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-005-6802-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02816.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-022-01420-w"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiad207"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2023.101146"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(04)00028-0"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2021.07.011"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0226"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erae023"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.TM117.000258"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.122796"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.096586"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70079"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.11.029"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants5030037"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac161"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01050"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab392"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.162479"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.880423"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.00379"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2021.100264"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.20.00964"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2017.01.003"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-018-9402-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2025.05.004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24076326"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcf037"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2010.03195.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23105375"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03212.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erj202"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2017.03.011"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2023.153950"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.140665"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcl029"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13635"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/fes3.379"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2023.06.007"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.01556"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiaf476"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20040292"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20253101"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-009-1047-7"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2023.09.007"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13601"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae112"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01010"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhag201"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7216"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13321"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac749"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006175"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhag087"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-021-00413-0"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.04.060"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erab448"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2019.10.009"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15532"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/156.3.1323"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.71124","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tpj.71124","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.71124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:25:58Z","timestamp":1792146358000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.71124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":63,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/tpj.71124"],"URL":"https:\/\/doi.org\/10.1111\/tpj.71124","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-05-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71124"}}