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In this work, we used the total protein approach (TPA), in which the total protein mass of a given proteomics sample is used as a calibrator for absolute protein quantification, to determine protein abundances during the\n                    <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>\n                    diurnal cycle. We use external, independently measured quantitative markers (metals, pigments) to assess the absolute protein abundances in unlabeled whole cell extracts. We calculate protein abundances in fg\u2009cell\n                    <jats:sup>\u22121<\/jats:sup>\n                    of 7322 Chlamydomonas proteins, 2266 of which were captured in every time point, including the major proteins involved in the light reactions, photoprotection, proteostasis, and fatty acid metabolism during a cell cycle. As expected, Rubisco large and small subunits are present in a 1:1 stoichiometry, with the large subunit being the most abundant protein in our data set, averaging 5.05\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    molecules per cell, reflecting 2.7% of the total protein mass. We noticed that PSII is the most abundant complex involved in the light reactions with 2.08\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    complexes per cell. PSI averages 1.75\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    complexes per cell and cytochrome\n                    <jats:italic>b<\/jats:italic>\n                    <jats:sub>6<\/jats:sub>\n                    <jats:italic>f<\/jats:italic>\n                    averages 0.77\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    complexes per cell. The TPA is a robust tool to study proteome dynamics quantitatively, while avoiding artifacts due to biochemical fractionation. Our proteome data set with an unprecedented temporal resolution is a valuable resource to assess protein abundances during the cell cycle in the reference alga Chlamydomonas.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71128","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:30:55Z","timestamp":1789684255000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Estimation of chloroplast macromolecular complex copy numbers and subunit stoichiometries during the\n                    <scp>\n                      <i>Chlamydomonas reinhardtii<\/i>\n                    <\/scp>\n                    cell cycle"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7487-8014","authenticated-orcid":false,"given":"Stefan","family":"Schmollinger","sequence":"first","affiliation":[{"name":"California Institute for Quantitative Biosciences, University of California  Berkeley California 94720 USA"},{"name":"Department of Chemistry and Biochemistry University of California  Los Angeles California 90095 USA"},{"name":"Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan 48824 USA"},{"name":"Department of Biochemistry and Molecular Biology Michigan State University  East Lansing Michigan 48824 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3420-1332","authenticated-orcid":false,"given":"Daniela","family":"Strenkert","sequence":"additional","affiliation":[{"name":"California Institute for Quantitative Biosciences, University of California  Berkeley California 94720 USA"},{"name":"Department of Chemistry and Biochemistry University of California  Los Angeles California 90095 USA"},{"name":"Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan 48824 USA"},{"name":"Department of Plant Biology Michigan State 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