{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T15:09:25Z","timestamp":1789744165999,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272682"],"award-info":[{"award-number":["32272682"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472708"],"award-info":[{"award-number":["32472708"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170315"],"award-info":[{"award-number":["32170315"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32570370"],"award-info":[{"award-number":["32570370"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) and its mediated protein persulfidation regulate fruit ripening. Fruit aroma, a key quality trait formed during ripening, largely determines consumer preferences. However, the mechanism by which H\n                    <jats:sub>2<\/jats:sub>\n                    S modulates aroma biosynthesis in pear remains unclear. This study reveals that H\n                    <jats:sub>2<\/jats:sub>\n                    S inhibited aroma biosynthesis in \u2018Nanguo\u2019\u00a0pear and downregulated the fatty acid desaturase gene\n                    <jats:italic>PuFAD8<\/jats:italic>\n                    . Transient overexpression and silencing assays confirmed that\n                    <jats:italic>PuFAD8<\/jats:italic>\n                    was required for linoleic acid synthesis, a key aroma precursor. Heterologous overexpression of PuFAD8 in tomato and its knockout in tomato further validated that\n                    <jats:italic>PuFAD8<\/jats:italic>\n                    promoted fruit aroma formation, while H\n                    <jats:sub>2<\/jats:sub>\n                    S suppressed this function. Transcriptome analysis identified transcription factors PuMYB21 and PuERF106 co\u2010expressed with\n                    <jats:italic>PuFAD8<\/jats:italic>\n                    . Dual\u2010luciferase and yeast one\u2010hybrid assays revealed that PuMYB21 bound and activated the\n                    <jats:italic>PuFAD8<\/jats:italic>\n                    promoter. Moreover, Y2H, luciferase complementation, pull\u2010down, and transient expression assays demonstrated that PuERF106 interacted with PuMYB21, synergistically enhancing aroma biosynthesis. Persulfidation at Cys162 of PuERF106 attenuated its interaction with PuMYB21, thereby inhibiting aromatic compound synthesis in pear fruit. Subsequently, the biological function of the PuERF106\u2010PuMYB21\u2010PuFAD8 molecular module was validated using stable transgenic pear callus lines and a panel of heterologous and endogenous transgenic tomato lines. This study reveals a novel regulatory mechanism where H\n                    <jats:sub>2<\/jats:sub>\n                    S\u2010mediated persulfidation suppresses pear aroma biosynthesis, providing a theoretical basis for genetic improvement of high\u2010quality pear.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71129","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:46:53Z","timestamp":1789742813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hydrogen sulfide\u2010mediated persulfidation of\n                    <scp>ERF106<\/scp>\n                    controls pear fruit aroma biosynthesis via disrupting the\n                    <scp>ERF106<\/scp>\n                    \u2010\n                    <scp>MYB21<\/scp>\n                    \u2010\n                    <scp>FAD8<\/scp>\n                    complex"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5916-6650","authenticated-orcid":false,"given":"Zhuoyan","family":"Xu","sequence":"first","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei 230009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1195-389X","authenticated-orcid":false,"given":"Kangdi","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei 230009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longfei","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei 230009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuwei","family":"Wei","sequence":"additional","affiliation":[{"name":"Shandong Institute of Pomology  Tai'an 271000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weisheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei 230009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huihui","family":"Song","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei 230009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7264-4153","authenticated-orcid":false,"given":"Gaifang","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei 230009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3980-1262","authenticated-orcid":false,"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology  Hefei 230009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.111740"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2020.01.004"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.31083\/fbl27736"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/978-1-62703-625-2_12","volume-title":"Synthetic biology","author":"Engler C.","year":"2013"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.106.4.1453"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/978-1-4939-3115-6_7","volume-title":"Plant signal transduction: methods and protocols","author":"Fantini E.","year":"2016"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiad155"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746\u20104811\u20101\u201013"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201061582\u20108"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae560"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.15309"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438\u2010018\u20100098\u2010y"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhaf273"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925\u20105214(02)00230\u20102"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jia.2024.12.036"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2022.112108"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2025.121900"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhaf275"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14675"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13851"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085524"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13761"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2024.108913"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2021.111560"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.127665"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870\u2010024\u201005594\u20104"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438\u2010020\u201000453\u20103"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407226200"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhac148"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.04.053"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2025.144493"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2025.1572093"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438\u2010021\u201000461\u2010x"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcz017"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438\u2010021\u201000590\u20103"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiad070"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010025\u201002273\u20104"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.70000"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/horticulturae11030310"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2025.1559012"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1039\/c5sc04818d"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24086946"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.127074"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiad100"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.18.00598"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00344\u2010021\u201010377\u20104"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2014.09.012"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2022788118"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1498658"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.71129","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tpj.71129","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tpj.71129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:46:55Z","timestamp":1789742815000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tpj.71129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/tpj.71129"],"URL":"https:\/\/doi.org\/10.1111\/tpj.71129","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71129"}}