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In this study, low nitrogen (N) stress induced the expression of\n                    <jats:italic>SnRK2.10<\/jats:italic>\n                    , which mitigated the adverse effects of N deficiency in apple plants.\n                    <jats:italic>SnRK2.10<\/jats:italic>\n                    \u2010overexpressing (OE) plants showed enhanced growth and higher nitrogen use efficiency (NUE), whereas\n                    <jats:italic>SnRK2.10<\/jats:italic>\n                    \u2010silenced (Ri) plants exhibited more severe growth inhibition and reduced NUE, compared with the wild\u2010type (WT) plants under low N conditions. The transcriptomic and proteomic analyses indicated that\n                    <jats:italic>SnRK2.10<\/jats:italic>\n                    regulates N metabolism and transport. It physically interacted with the nitrate transporter NRT2.5 and the ammonium transporter AMT1.1. Consistently, OE plants displayed higher NO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    and NH\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>+<\/jats:sup>\n                    uptake rates than WT and Ri plants under low N stress. Functional analyses using transgenic apple lines with different expression levels of\n                    <jats:italic>NRT2.5<\/jats:italic>\n                    or\n                    <jats:italic>AMT1.1<\/jats:italic>\n                    demonstrated that both transporters positively regulate growth and NUE in apple plants' response to low N stress. Co\u2010expression of\n                    <jats:italic>SnRK2.10<\/jats:italic>\n                    with either\n                    <jats:italic>NRT2.5<\/jats:italic>\n                    or\n                    <jats:italic>AMT1.1<\/jats:italic>\n                    effectively altered plant growth under low N stress. Collectively, these results indicate that\n                    <jats:italic>SnRK2.10<\/jats:italic>\n                    positively regulates plant growth and NUE under low N stress, probably by promoting N uptake through direct interaction with key N\u00a0transporters.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71139","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:49:31Z","timestamp":1791269371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>SnRK2.10<\/i>\n                    in apple positively regulates the nitrogen use efficiency via modulating the nitrogen uptake"],"prefix":"10.1111","volume":"127","author":[{"given":"Ying","family":"Ye","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas\/Shaanxi Key Laboratory of Apple, College of Horticulture Northwest A&amp;F University  Yangling 712100 Shaanxi 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