{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:40:14Z","timestamp":1792618814271,"version":"4.5.0"},"reference-count":84,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15F P15390"],"award-info":[{"award-number":["15F P15390"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP19H02921"],"award-info":[{"award-number":["JP19H02921"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","award":["26001AB"],"award-info":[{"award-number":["26001AB"]}],"id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","award":["IVG1005"],"award-info":[{"award-number":["IVG1005"]}],"id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","award":["DIT1003"],"award-info":[{"award-number":["DIT1003"]}],"id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","award":["DIT2002"],"award-info":[{"award-number":["DIT2002"]}],"id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Soybean (\n                    <jats:italic>Glycine max<\/jats:italic>\n                    (L.) Merr.) produces triterpenoid saponins, called soyasaponins, which are categorised as 2,3\u2010dihydro\u20102,5\u2010dihydroxy\u20106\u2010methyl\u20104\n                    <jats:italic>H<\/jats:italic>\n                    \u2010pyran\u20104\u2010one (DDMP) saponins and group A saponins based on the chemical modifications at the C\u201022 position of their triterpene skeleton. These C\u201022 modifications confer different biological functions. While most genes involved in soyasaponin biosynthesis have been identified, the enzymes responsible for conjugation of DDMP or arabinose to the C\u201022 hydroxyl group remain unclear. This study aimed to identify the enzymes involved in these modifications. Co\u2010expression analysis using known saponin biosynthetic genes identified three candidate\n                    <jats:italic>UGT<\/jats:italic>\n                    genes. Among them,\n                    <jats:italic>UGT73K5<\/jats:italic>\n                    was essential for DDMP saponin biosynthesis in soybean, as\n                    <jats:italic>ugt73k5<\/jats:italic>\n                    mutants lacked these compounds. Similarly, the orthologous gene in\n                    <jats:italic>Lotus japonicus<\/jats:italic>\n                    ,\n                    <jats:italic>UGT73K6<\/jats:italic>\n                    , was required for DDMP saponin biosynthesis, and the\n                    <jats:italic>ugt73k6<\/jats:italic>\n                    defect was complemented by soybean\n                    <jats:italic>UGT73K5<\/jats:italic>\n                    . In addition,\n                    <jats:italic>UGT73BU1<\/jats:italic>\n                    , a\u00a0homologous gene not co\u2010expressed with known saponin biosynthetic genes, was identified as a C\u201022 arabinosyltransferase involved in group A saponin biosynthesis. In\n                    <jats:italic>L. japonicus<\/jats:italic>\n                    ,\n                    <jats:italic>UGT73K6<\/jats:italic>\n                    is genomically co\u2010localised and co\u2010expressed with a cytochrome P450 gene responsible for C\u201022 hydroxylation, forming a conserved biosynthetic gene cluster. This organisation suggests coordinated biosynthetic modules underlying DDMP saponin production in legumes.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71149","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T09:09:01Z","timestamp":1792141741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A modular\n                    <scp>UDP<\/scp>\n                    \u2010glycosyltransferase and cytochrome\n                    <scp>P450<\/scp>\n                    system for soyasaponin C\u201022 modification"],"prefix":"10.1111","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2937-6832","authenticated-orcid":false,"given":"Susumu","family":"Hiraga","sequence":"first","affiliation":[{"name":"Institute of Crop Science National Agriculture and Food Research Organization (NARO)  Tsukuba Ibaraki 305\u20108518 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