{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:50:04Z","timestamp":1792155004405,"version":"4.4.0"},"reference-count":74,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100020068","name":"Topconsortium voor Kennis en Innovatie","doi-asserted-by":"publisher","award":["TU202208"],"award-info":[{"award-number":["TU202208"]}],"id":[{"id":"10.13039\/501100020068","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Chemically inducible gene expression systems provide temporal control of transgene activation and therefore represent powerful and widely used tools in plant research. When driven by a cell\u2010type\u2010specific promoter, these systems additionally offer spatial control. However, continued transgene activity is necessarily linked to the preservation of that particular cell type, which might be lost as cells (de)differentiate. To overcome this, we present Tissue\u2010specific Recombination\u2010Activated Clonal and Inducible Transcription (TRAC\u2010IT), a two\u2010step system that uncouples inducible gene expression from cell identity. In the first step, a tissue\u2010specific expressed CRE\u2010GR recombinase is activated upon dexamethasone application, triggering loxP recombination and thereby generating tissue\u2010specific clones of cells expressing\n                    <jats:italic>YFP<\/jats:italic>\n                    and\n                    <jats:italic>XVE<\/jats:italic>\n                    . YFP marks the clone and the XVE\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010activator allows inducible expression of downstream genes within the clone. In the second step, a subsequent \u03b2\u2010estradiol application activates XVE to induce transgene expression exclusively within these YFP\u2010marked clones. Because the inducibility of the transgenes becomes genomically anchored, it is maintained upon cell division and\/or identity change. TRAC\u2010IT is compatible with Golden Gate cloning, providing simple variation of tissue\u2010specific promoters and transgenes. We show that TRAC\u2010IT supports inducible and controlled tissue\u2010specific clone formation, and that transgenes can be specifically induced within these clones. We employed TRAC\u2010IT to demonstrate that epidermal cells in the root meristem can be persuaded to regenerate somatic embryos through clone\u2010based induction of\n                    <jats:italic>PLT1<\/jats:italic>\n                    and\n                    <jats:italic>WOX5<\/jats:italic>\n                    . Thus, TRAC\u2010IT offers a versatile tool that provides new technical possibilities to explore the mechanisms driving plant development in a stable tissue\u2010specific fashion.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71151","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T07:43:22Z","timestamp":1790581402000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tissue\u2010specific recombination\u2010activated clonal and inducible transcription (\n                    <scp>TRAC<\/scp>\n                    \u2010\n                    <scp>IT<\/scp>\n                    ) enables controlled gene activation in stably marked tissues"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9039-9260","authenticated-orcid":false,"given":"Tristan","family":"Wijsman","sequence":"first","affiliation":[{"name":"Cell\u2010and Developmental Biology, Cluster Plant Developmental Biology Wageningen University &amp; Research  Droevendaalsesteeg 1 6708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6575-4724","authenticated-orcid":false,"given":"Jana","family":"Wittmer","sequence":"additional","affiliation":[{"name":"Cell\u2010and Developmental Biology, Cluster Plant Developmental Biology Wageningen University &amp; Research  Droevendaalsesteeg 1 6708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9934-5897","authenticated-orcid":false,"given":"Robert","family":"Winter","sequence":"additional","affiliation":[{"name":"Cell\u2010and Developmental Biology, Cluster Plant Developmental Biology Wageningen University &amp; Research  Droevendaalsesteeg 1 6708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5131-9383","authenticated-orcid":false,"given":"Teun","family":"Bruins","sequence":"additional","affiliation":[{"name":"Cell\u2010and Developmental Biology, Cluster Plant Developmental Biology Wageningen University &amp; Research  Droevendaalsesteeg 1 6708 PB Wageningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjan","family":"van Zeijl","sequence":"additional","affiliation":[{"name":"Rijk Zwaan Breeding B.V.  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