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CYP73 encodes the second enzyme in the core phenylpropanoid pathway and is the first enzyme family in this pathway so far exclusively found in land plants and not in any water\u2010living sister clades, like the streptophyte algae. That makes CYP73 a prime candidate for investigations into necessary adaptations during terrestrialization. This study aims to support our knowledge on the evolution of this pathway by characterizing the enzymes translated from (putative) CYP73 genes from the bryophytes\n                    <jats:italic>Anthoceros agrestis<\/jats:italic>\n                    ,\n                    <jats:italic>Marchantia polymorpha<\/jats:italic>\n                    , and\n                    <jats:italic>Physcomitrium patens<\/jats:italic>\n                    and CYP98 genes from\n                    <jats:italic>Marchantia polymorpha<\/jats:italic>\n                    and\n                    <jats:italic>Physcomitrium patens<\/jats:italic>\n                    . Furthermore, this insight into the function of bryophyte CYP73 informed a more specific search for potential CYP73 candidates in the streptophyte alga\n                    <jats:italic>Chara braunii<\/jats:italic>\n                    . The possibility that land plant CYP73 activity originated in streptophyte algae would be greatly supported if rudimentary CYP73 activity could be found in native\n                    <jats:italic>Chara<\/jats:italic>\n                    candidate genes or induced by slight mutations in inactive\n                    <jats:italic>Chara<\/jats:italic>\n                    enzymes. However, no such mutation led to product formation by enzymes encoded by\n                    <jats:italic>Chara braunii<\/jats:italic>\n                    candidate genes. The bryophyte CYP98 enzymes prefer 4\u2010coumaroyl amides as substrates, though 4\u2010coumaroyl esters are generally the common substrates of tracheophyte CYP98 enzymes. This shows a major difference in functionality. Thus, this study provides a comparative characterization of CYP genes from model bryophytes that are canonically associated with phenylpropanoid metabolism.\n                  <\/jats:p>","DOI":"10.1111\/tpj.71152","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:32:18Z","timestamp":1791441138000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytochrome\n                    <scp>P450<\/scp>\n                    enzymes in the phenylpropanoid pathway:\n                    <scp>CYP73<\/scp>\n                    and\n                    <scp>CYP98<\/scp>\n                    candidate genes in\n                    <i>Chara braunii<\/i>\n                    ,\n                    <i>Anthoceros agrestis<\/i>\n                    ,\n                    <i>Marchantia polymorpha<\/i>\n                    and\n                    <i>Physcomitrium patens<\/i>"],"prefix":"10.1111","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4770-2541","authenticated-orcid":false,"given":"Christoph E. 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