{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:42:18Z","timestamp":1788871338494,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T00:00:00Z","timestamp":1350345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["GM-49217"],"award-info":[{"award-number":["GM-49217"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      \u2018\n                      <jats:italic>G<\/jats:italic>\n                      yrating\u2010\u2019 or \u2018\n                      <jats:italic>G<\/jats:italic>\n                      \u2019\u2010clathrin are coated endocytic structures located near peripheral sorting endosomes (\n                      <jats:styled-content style=\"fixed-case\">SEs<\/jats:styled-content>\n                      ), which exhibit highly dynamic but localized movements when visualized by live\u2010cell microscopy. They have been implicated in recycling of transferrin from the sorting endosome directly to the cell surface, but there is no information about their formation or regulation. We show here that G\u2010clathrin comprise a minority of clathrin\u2010coated structures in the cell periphery and are brefeldin A (\n                      <jats:styled-content style=\"fixed-case\">BFA<\/jats:styled-content>\n                      )\u2010resistant. Arf6\u2010\n                      <jats:styled-content style=\"fixed-case\">GTP<\/jats:styled-content>\n                      substantially increases G\u2010clathrin levels, probably by lengthening coated bud lifetimes as suggested by photobleaching and photoactivation results, and an Arf6(\n                      <jats:styled-content style=\"fixed-case\">Q67L<\/jats:styled-content>\n                      )\u2010\n                      <jats:styled-content style=\"fixed-case\">GTP<\/jats:styled-content>\n                      mutant bearing an internal\n                      <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                      tag can be directly visualized in G\u2010clathrin structures in live cells. Upon\n                      <jats:styled-content style=\"fixed-case\">siRNA<\/jats:styled-content>\n                      \u2010mediated depletion of Arf6 or expression of Arf6(\n                      <jats:styled-content style=\"fixed-case\">T27N<\/jats:styled-content>\n                      ), G\u2010clathrin levels rise and are primarily Arf1\u2010dependent, yet still\n                      <jats:styled-content style=\"fixed-case\">BFA<\/jats:styled-content>\n                      \u2010resistant. However,\n                      <jats:styled-content style=\"fixed-case\">BFA<\/jats:styled-content>\n                      \u2010sensitive increased G\u2010clathrin levels are observed upon acute incubation with cytohesin inhibitor\n                      <jats:styled-content style=\"fixed-case\">SecinH3<\/jats:styled-content>\n                      , indicating a shift in\n                      <jats:styled-content style=\"fixed-case\">GEF<\/jats:styled-content>\n                      usage. Depletion of both Arf6 and Arf1 abolishes G\u2010clathrin, and results in partial inhibition of fast transferrin recycling consistent with the latter's participation in this pathway. Collectively, these results demonstrate that the dynamics of G\u2010clathrin primarily requires completion of the Arf6 guanine nucleotide cycle, but can be regulated by multiple Arf and\n                      <jats:styled-content style=\"fixed-case\">GEF<\/jats:styled-content>\n                      proteins, reflecting both overlapping mechanisms operative in their regulation and the complexity of processes involved in endosomal sorting.\n                    <\/jats:bold>\n                  <\/jats:p>","DOI":"10.1111\/tra.12014","type":"journal-article","created":{"date-parts":[[2012,9,21]],"date-time":"2012-09-21T14:15:08Z","timestamp":1348236908000},"page":"97-106","source":"Crossref","is-referenced-by-count":14,"title":["Arf6 Regulation of Gyrating\u2010Clathrin"],"prefix":"10.1111","volume":"14","author":[{"given":"Yi","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Thomas Jefferson University  Philadelphia PA 19107 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Thomas Jefferson University  Philadelphia PA 19107 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H.","family":"Keen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Thomas Jefferson University  Philadelphia PA 19107 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1985"},{"key":"e_1_2_6_3_1","first-page":"99","article-title":"The early endosome: a busy sorting station for proteins at the crossroads","volume":"25","author":"Jovic M","year":"2010","journal-title":"Histol Histopathol"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5362.431"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.4.809"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-10-0525"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.132.1.21"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.141.3.611"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-07-0380"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.003020"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00819.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.05.007"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1910"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3117"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2005.04.005"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2427"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.01.015"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.05.006"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90124-U"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.02-07-0109"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.149.1.81"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.149.1.67"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20020428"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M600149200"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.128.6.1003"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.3.603"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.28.20040"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.4.1241"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00299-9"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01090"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2007.11.032"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2010.01054.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.02.034"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05415"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.1.23"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200104085"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E02-06-0353"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02978"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074952"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606998200"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1315"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2006.00499.x"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00250.2003"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.1.7"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108399200"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401753101"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/384481a0"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011577200"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M513723200"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-12-1042"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.261800"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-11-0998"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2007.01723.x"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1109\/TMI.2009.2033991"}],"container-title":["Traffic"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftra.12014","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tra.12014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T19:38:14Z","timestamp":1698521894000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tra.12014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,16]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1111\/tra.12014"],"URL":"https:\/\/doi.org\/10.1111\/tra.12014","archive":["Portico"],"relation":{},"ISSN":["1398-9219","1600-0854"],"issn-type":[{"value":"1398-9219","type":"print"},{"value":"1600-0854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,16]]}}}