{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:49:07Z","timestamp":1787237347630,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T00:00:00Z","timestamp":1376524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2013,10]]},"abstract":"\n \n TRAPP<\/jats:styled-content>\n is a multisubunit complex that functions in membrane traffic. Mutations in the mammalian\n TRAPP<\/jats:styled-content>\n protein\n C2<\/jats:styled-content>\n are linked to the skeletal disorder spondyloepiphyseal dysplasia tarda (\n SEDT<\/jats:styled-content>\n ) that is thought to arise from an inability to secrete procollagen from the endoplasmic reticulum. Here, we show that\n C2<\/jats:styled-content>\n binds to the\n SNARE<\/jats:styled-content>\n protein Syntaxin 5 and this interaction is weakened by an\n SEDT<\/jats:styled-content>\n \u2010causing missense mutation (\n D47Y<\/jats:styled-content>\n ). Interestingly, the equivalent mutation (\n D46Y<\/jats:styled-content>\n ) in the yeast\n C2<\/jats:styled-content>\n homolog Trs20p does not block anterograde traffic but did affect endocytosis. The\n \n trs20D46Y<\/jats:italic>\n <\/jats:styled-content>\n mutation interfered with the interaction between Trs20p and Trs85p (\n TRAPP III<\/jats:styled-content>\n \u2010specific subunit), Trs120p and Trs130p (\n TRAPP II<\/jats:styled-content>\n \u2010specific subunits). Size exclusion chromatography suggested that this yeast mutation destabilized the\n TRAPP III<\/jats:styled-content>\n complex that is involved in autophagy. We further show that this mutation blocks both the selective cytosol\u2010to\u2010vacuole (cvt) pathway as well as non\u2010selective autophagy. We demonstrate that the apparent molecular size of the\n TRAPP III<\/jats:styled-content>\n complex is dependent upon membranes, and that the presence of\n TRAPP III<\/jats:styled-content>\n is dependent upon Atg9p. Finally, we demonstrate that lipidated Bet3p is enriched in\n TRAPP III<\/jats:styled-content>\n and that lipidation increases the efficiency of autophagy. Our study suggests that Trs20p acts as an adaptor for Trs85p and Trs120p and reveals complexities in\n TRAPP III<\/jats:styled-content>\n assembly and function. The implications of\n C2D47Y<\/jats:styled-content>\n in\n SEDT<\/jats:styled-content>\n are discussed\n <\/jats:bold>\n .\n <\/jats:p>","DOI":"10.1111\/tra.12095","type":"journal-article","created":{"date-parts":[[2013,7,30]],"date-time":"2013-07-30T13:54:31Z","timestamp":1375192471000},"page":"1091-1104","source":"Crossref","is-referenced-by-count":30,"title":["A\n trs20<\/i>\n Mutation That Mimics an\n SEDT<\/scp>\n \u2010Causing Mutation Blocks Selective and Non\u2010Selective Autophagy: A Model for\n TRAPP III<\/scp>\n Organization"],"prefix":"10.1111","volume":"14","author":[{"given":"Stephanie","family":"Brunet","sequence":"first","affiliation":[{"name":"Department of Biology Concordia University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nassim","family":"Shahrzad","sequence":"additional","affiliation":[{"name":"Department of Biology Concordia University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djenann","family":"Saint\u2010Dic","sequence":"additional","affiliation":[{"name":"Department of Biology Concordia University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartley","family":"Dutczak","sequence":"additional","affiliation":[{"name":"Department of Biology Concordia University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Sacher","sequence":"additional","affiliation":[{"name":"Department of Biology Concordia University Montreal Quebec Canada"},{"name":"Department of Anatomy and Cell Biology McGill University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2000.011104.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.31103.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.04.005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05527"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09969"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.12.4403"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.151.2.289"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.6.12.1769"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00190-3"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000063107"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2009.00895.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2011.01181.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200505145"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12024"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501701200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1121299109"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.112.139378"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.3.1015"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.131.3.583"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2010.01031.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90433-3"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0004.2003.00132.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1086\/320594"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1224947"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2009.00906.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(03)00819-9"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101584200"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207436200"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062950"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-07-0535"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90241-0"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2008.02.021"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.04.049"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.09.029"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023350"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00127-2"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.4161\/cl.19414"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.22.12304"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00373-8"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2009.01299.x"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb871"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600565"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603513103"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.411454"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200408124"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-10-0894"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2005.06.013"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00323-1"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0358-y"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1914"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-12-0876"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-01-0004"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e10-11-0873"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.2.287"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508885102"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI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