{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:31:14Z","timestamp":1787243474059,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,14]],"date-time":"2014-01-14T00:00:00Z","timestamp":1389657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2014,3]]},"abstract":"\n The modular\n TRAPP<\/jats:styled-content>\n complex acts as a guanine\u2010nucleotide exchange factor (\n GEF<\/jats:styled-content>\n ) for Ypt\/Rab\n GTPases<\/jats:styled-content>\n . Whereas\n TRAPP<\/jats:styled-content>\n I and\n TRAPP II<\/jats:styled-content>\n regulate the exocytic pathway,\n TRAPP III<\/jats:styled-content>\n functions in autophagy. The\n TRAPP<\/jats:styled-content>\n subunit Trs20 is not required for assembly of core\n TRAPP<\/jats:styled-content>\n or its Ypt1\n GEF<\/jats:styled-content>\n activity. Interestingly, mutations in the human functional ortholog of Trs20, Sedlin, cause spondyloepiphyseal dysplasia tarda (\n SEDT<\/jats:styled-content>\n ), a cartilage\u2010specific disorder. We have shown that Trs20 is required for\n TRAPP II<\/jats:styled-content>\n assembly and identified a\n SEDT<\/jats:styled-content>\n \u2010linked mutation, Trs20\u2010\n D46Y<\/jats:styled-content>\n , which causes a defect in this process. Here we show that Trs20 is also required for assembly of\n TRAPP III<\/jats:styled-content>\n at the pre\u2010autophagosomal structure (\n PAS<\/jats:styled-content>\n ). First, recombinant Trs85, a\n TRAPP III<\/jats:styled-content>\n \u2010specific subunit, associates with\n TRAPP<\/jats:styled-content>\n only in the presence of Trs20, but not Trs20\u2010\n D46Y<\/jats:styled-content>\n mutant protein. Second, a\n TRAPP<\/jats:styled-content>\n complex with Ypt1\n GEF<\/jats:styled-content>\n activity co\u2010precipitates with Trs85 from wild type, but not\n trs20ts<\/jats:italic>\n mutant, cell lysates. Third, live\u2010cell colocalization analysis indicates that Trs85 recruits core\n TRAPP<\/jats:styled-content>\n to the\n PAS<\/jats:styled-content>\n via the linker protein Trs20. Finally,\n trs20ts<\/jats:italic>\n mutant cells are defective in selective and non\u2010selective autophagy. Together, our results show that Trs20 plays a role as an adaptor in the assembly of\n TRAPP II<\/jats:styled-content>\n and\n TRAPP III<\/jats:styled-content>\n complexes, and the\n SEDT<\/jats:styled-content>\n \u2010linked mutation causes a defect in both processes.\n <\/jats:p>\n \n \n <\/jats:p>","DOI":"10.1111\/tra.12145","type":"journal-article","created":{"date-parts":[[2013,12,10]],"date-time":"2013-12-10T12:22:43Z","timestamp":1386678163000},"page":"327-337","source":"Crossref","is-referenced-by-count":30,"title":["Trs20 is Required for\n TRAPP III<\/scp>\n Complex Assembly at the\n PAS<\/scp>\n and its Function in Autophagy"],"prefix":"10.1111","volume":"15","author":[{"given":"David","family":"Taussig","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Illinois at Chicago 900 South Ashland Avenue Chicago IL 60612 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanna","family":"Lipatova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Illinois at Chicago 900 South Ashland Avenue Chicago IL 60612 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nava","family":"Segev","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Illinois at Chicago 900 South Ashland Avenue Chicago IL 60612 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.09.029"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12065"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2009.00988.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00833.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000063107"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2009.02.006"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1193497"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2708"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-00302-8_1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1121299109"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e13-05-0269"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/11976"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1224947"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023350"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501701200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.1.1.1512"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12024"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E07-03-0221"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.411454"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1489"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19970915)13:11<1065::AID-YEA159>3.0.CO;2-K"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.7.2367"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(08)03201-1"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90648-A"}],"container-title":["Traffic"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftra.12145","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tra.12145","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T08:50:31Z","timestamp":1695977431000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tra.12145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,14]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1111\/tra.12145"],"URL":"https:\/\/doi.org\/10.1111\/tra.12145","archive":["Portico"],"relation":{},"ISSN":["1398-9219","1600-0854"],"issn-type":[{"value":"1398-9219","type":"print"},{"value":"1600-0854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,14]]}}}