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While budding yeast has clear homologues of all four <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>\u20102 subunits which form a complex and localize to endocytic sites <jats:italic>in vivo<\/jats:italic>, the function of yeast <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>\u20102 has remained enigmatic. Here, we demonstrate that <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>\u20102 is required for hyphal growth in <jats:italic>Candida albicans<\/jats:italic> and polarized cell responses in <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. Deletion of <jats:italic><jats:styled-content style=\"fixed-case\">APM4<\/jats:styled-content><\/jats:italic>, the cargo\u2010binding mu subunit of <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>\u20102, causes defects in pseudohyphal growth, generation of a mating projection and the cell wall damage response. In an <jats:italic>apm4<\/jats:italic> null mutant, the cell wall stress sensor Mid2 is unable to relocalize to the tip of a mating projection following pheromone addition, or to the mother bud neck in response to cell wall damage. A direct binding interaction between Mid2 and the mu homology domain of Apm4 further supports a model in which <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>\u20102 binds Mid2 to facilitate its internalization and relocalization in response to specific signals. Thus, Mid2 is the first cargo for <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>\u20102 identified in yeast. We propose that endocytic recycling of Mid2 and other components is required for polarized cell responses ensuring cell wall deposition and is tightly monitored during cell growth.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12155-gra-0001.gif\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12155","type":"journal-article","created":{"date-parts":[[2014,1,24]],"date-time":"2014-01-24T14:29:45Z","timestamp":1390573785000},"page":"546-557","source":"Crossref","is-referenced-by-count":41,"title":["Yeast Endocytic Adaptor <scp>AP<\/scp>\u20102 Binds the Stress Sensor Mid2 and Functions in Polarized Cell Responses"],"prefix":"10.1111","volume":"15","author":[{"given":"Bernardo","family":"Chapa\u2010y\u2010Lazo","sequence":"first","affiliation":[{"name":"Department of Biomedical Science University of Sheffield Sheffield S10 2TN UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen G.","family":"Allwood","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science University of Sheffield Sheffield S10 2TN UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona I.","family":"Smaczynska\u2010de Rooij","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science University of Sheffield Sheffield S10 2TN UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary L.","family":"Snape","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science University of Sheffield Sheffield S10 2TN UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn R.","family":"Ayscough","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science University of Sheffield Sheffield S10 2TN UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00735-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00500a011"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.2.341"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5392.1327"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.05.006"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07422"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2006-0044OC"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00240-8"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.21.9318-9323.2005"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-05-0452"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010597"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.08.006"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.11.3643"},{"key":"e_1_2_6_15_1","first-page":"3347","article-title":"Mannoprotein of the yeast cell wall as primary receptor for the killer toxin of S. cerevisiae strain 28","volume":"133","author":"Schmitt M","year":"1987","journal-title":"J Gen Microbiol"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.5.2192-2196.1988"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.8.1615"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.5.1833"},{"key":"e_1_2_6_19_1","unstructured":"Neurospora crassa sequencing Project. 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