{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:14:44Z","timestamp":1790705684337,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T00:00:00Z","timestamp":1435536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01.AI095175-01"],"award-info":[{"award-number":["R01.AI095175-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2010-1141"],"award-info":[{"award-number":["PICT 2010-1141"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>\n                    Dengue viruses cause the most important human viral disease transmitted by mosquitoes. In recent years, a great deal has been learned about molecular details of dengue virus genome replication; however, little is known about genome encapsidation and the functions of the viral capsid protein. During infection, dengue virus capsid progressively accumulates around lipid droplets (LDs) by an unknown mechanism. Here, we examined the process by which the viral capsid is transported from the endoplasmic reticulum (\n                    <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>\n                    ) membrane, where the protein is synthesized, to LDs. Using different methods of intervention, we found that the\n                    <jats:styled-content style=\"fixed-case\">GBF1<\/jats:styled-content>\n                    \u2010Arf1\/Arf4\u2010\n                    <jats:styled-content style=\"fixed-case\">COPI<\/jats:styled-content>\n                    pathway is necessary for capsid transport to LDs, while the process is independent of both\n                    <jats:styled-content style=\"fixed-case\">COPII<\/jats:styled-content>\n                    components and Golgi integrity. The transport was sensitive to Brefeldin A, while a drug resistant form of\n                    <jats:styled-content style=\"fixed-case\">GBF1<\/jats:styled-content>\n                    was sufficient to restore capsid subcellular distribution in infected cells. The mechanism by which LDs gain or lose proteins is still an open question. Our results support a model in which the virus uses a non\u2010canonical function of the\n                    <jats:styled-content style=\"fixed-case\">COPI<\/jats:styled-content>\n                    system for capsid accumulation on LDs, providing new ideas for antiviral strategies.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12305-gra-0001.png\" xlink:title=\"image\"\/>\n                  <\/jats:p>","DOI":"10.1111\/tra.12305","type":"journal-article","created":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T03:46:18Z","timestamp":1433130378000},"page":"962-977","source":"Crossref","is-referenced-by-count":80,"title":["Dengue Virus Uses a Non\u2010Canonical Function of the Host\n                    <scp>GBF1<\/scp>\n                    \u2010Arf\u2010\n                    <scp>COPI<\/scp>\n                    System for Capsid Protein Accumulation on Lipid Droplets"],"prefix":"10.1111","volume":"16","author":[{"given":"Nestor G.","family":"Iglesias","sequence":"first","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir\u2010CONICET  Avenida Patricias Argentinas 435 Buenos Aires 1405 Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Mondotte","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir\u2010CONICET  Avenida Patricias Argentinas 435 Buenos Aires 1405 Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Byk","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir\u2010CONICET  Avenida Patricias Argentinas 435 Buenos Aires 1405 Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico A.","family":"De Maio","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir\u2010CONICET  Avenida Patricias Argentinas 435 Buenos Aires 1405 Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo M.","family":"Samsa","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir\u2010CONICET  Avenida Patricias Argentinas 435 Buenos Aires 1405 Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda\u2010CONICET Departamento de Bioqu\u00edmica Cl\u00ednica, Facultad de Ciencias Qu\u00edmicas, UNC  5000 C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea V.","family":"Gamarnik","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir\u2010CONICET  Avenida Patricias Argentinas 435 Buenos Aires 1405 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