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Distinct isoforms of <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> are found in different tissues and subcellular localization patterns. For example, <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> \u03b11 is widely expressed, <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> \u03b13 is enriched in neurons and <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> \u03b14 is a testes\u2010specific isoform found in sperm flagella. In some tissues, ankyrin, a key component of the membrane cytoskeleton, can regulate the trafficking of <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content>. In the retina, <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> and ankyrin\u2010B are expressed in multiple cell types and immunostaining for each is striking in the synaptic layers. Labeling for <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> is also prominent along the inner segment plasma membrane (ISPM) of photoreceptors. <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> co\u2010immunoprecipitates with ankyrin\u2010B, but on a subcellular level colocalization of these two proteins varies dependent on the cell type. We used transgenic <jats:italic>Xenopus laevis<\/jats:italic> tadpoles to evaluate the subcellular  trafficking of <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> in photoreceptors. <jats:styled-content style=\"fixed-case\">GFP\u2010NKA<\/jats:styled-content> \u03b13 and \u03b11 are localized to the ISPM, but \u03b14 is localized to outer segments (OSs). We identified a <jats:styled-content style=\"fixed-case\">VxP<\/jats:styled-content> motif responsible for the OS targeting by using a series of chimeric and mutant <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> constructs. This motif is similar to previously identified ciliary targeting motifs. Given the structural similarities between OSs and flagella, our findings shed light on the subcellular targeting of this testes\u2010specific <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> isoform.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12332-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12332","type":"journal-article","created":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T10:21:35Z","timestamp":1442398895000},"page":"1239-1253","source":"Crossref","is-referenced-by-count":8,"title":["Identification of a <scp>VxP<\/scp> Targeting Signal in the Flagellar Na<sup>+<\/sup>\/K<sup>+<\/sup>\u2010<scp>ATPase<\/scp>"],"prefix":"10.1111","volume":"16","author":[{"given":"Joseph G.","family":"Laird","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Carver College of Medicine University of Iowa  Iowa City IA 52242 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Ophthalmology West Virginia University School of Medicine and West Virginia University Eye Institute  Morgantown WV 26506 USA"},{"name":"Current address: Section for Translational Research in Retina &amp; Macular Degeneration National Institute on Deafness and Other Communication Disorders, NIH  Bethesda MD 20892 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxim","family":"Sokolov","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology West Virginia University School of Medicine and West Virginia University Eye Institute  Morgantown WV 26506 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila A.","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Carver College of Medicine University of Iowa  Iowa City IA 52242 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