{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:35:00Z","timestamp":1789904100453,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T00:00:00Z","timestamp":1446422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["075880"],"award-info":[{"award-number":["075880"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["100140"],"award-info":[{"award-number":["100140"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["097024\/B\/11\/Z"],"award-info":[{"award-number":["097024\/B\/11\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000648","name":"King's College Cambridge, University of Cambridge","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000648","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2015,12]]},"abstract":"\n Cytotoxic T\u2010lymphocytes (\n CTL<\/jats:styled-content>\n ) kill their targets by cytolytic granule secretion at the immunological synapse. The Sec\/Munc protein, Munc18\u20102, and its binding partner Syntaxin 11 (\n STX11<\/jats:styled-content>\n ) are both required for granule secretion, with mutations in either leading to the primary immunodeficiency, Familial Haemophagocytic Lymphohistiocytosis (\n FHL4<\/jats:styled-content>\n and 5). Understanding how Munc18\u20102 and\n STX11<\/jats:styled-content>\n function in\n CTL<\/jats:styled-content>\n has been hampered by not knowing the endogenous localization of these proteins. Using a novel\n FHL5<\/jats:styled-content>\n Munc18\u20102 mutation that results in loss of protein, cytotoxicity and degranulation together with\n CTL<\/jats:styled-content>\n from an\n FHL4<\/jats:styled-content>\n patient lacking\n STX11<\/jats:styled-content>\n , enabled us to localize endogenous\n STX11<\/jats:styled-content>\n and Munc18\u20102 in\n CTL<\/jats:styled-content>\n . Munc18\u20102 localized predominantly to cytolytic granules with low levels associated with the plasma membrane where\n STX11<\/jats:styled-content>\n localized. Importantly, while Munc18\u20102 localization is unaffected by the absence of\n STX11<\/jats:styled-content>\n in\n FHL4 CTL<\/jats:styled-content>\n ,\n STX11<\/jats:styled-content>\n is lost from the plasma membrane in\n FHL5 CTL<\/jats:styled-content>\n lacking Munc18\u20102. These findings support a role for Munc18\u20102 in chaperoning\n STX11<\/jats:styled-content>\n to the plasma membrane where the final fusion events involved in secretion occur.\n <\/jats:p>\n \n \n <\/jats:p>","DOI":"10.1111\/tra.12337","type":"journal-article","created":{"date-parts":[[2015,9,25]],"date-time":"2015-09-25T04:45:50Z","timestamp":1443156350000},"page":"1330-1341","source":"Crossref","is-referenced-by-count":34,"title":["Munc18\u20102 is required for Syntaxin 11 Localization on the Plasma Membrane in\u00a0Cytotoxic T\u2010Lymphocytes"],"prefix":"10.1111","volume":"16","author":[{"given":"Nele M.G.","family":"Dieckmann","sequence":"first","affiliation":[{"name":"Cambridge Institute for Medical Research, Cambridge Biomedical Campus University of Cambridge Cambridge CB2 0XY United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Hackmann","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research, Cambridge Biomedical Campus University of Cambridge Cambridge CB2 0XY United Kingdom"},{"name":"Current address: Biochemistry Center Heidelberg University Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Aric\u00f2","sequence":"additional","affiliation":[{"name":"Azienda Sanitaria Provinciale 7 Piazza Igea 1 I\u201097100 Ragusa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian M.","family":"Griffiths","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research, Cambridge Biomedical Campus University of Cambridge Cambridge CB2 0XY United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.08.013"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi076"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI40732"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2009.09.005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2014.03.002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00167"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.18.3323"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e11-02-0135"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201142343"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-02-074468"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.6.3397"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201344011"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-11-610816"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1431"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.11.5857"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.22.13022"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00109"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1301277"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.235366"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.19.110701.155609"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11320"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201411093"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0658-07.2007"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098900"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1313474110"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-12-398958"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00467"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01280.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141915"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00216"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.05.023"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-08-374199"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.161653"}],"container-title":["Traffic"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftra.12337","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tra.12337","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T18:11:15Z","timestamp":1696183875000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tra.12337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,2]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/tra.12337"],"URL":"https:\/\/doi.org\/10.1111\/tra.12337","archive":["Portico"],"relation":{},"ISSN":["1398-9219","1600-0854"],"issn-type":[{"value":"1398-9219","type":"print"},{"value":"1600-0854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,2]]}}}