{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:24:15Z","timestamp":1790763855260,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,4,21]],"date-time":"2016-04-21T00:00:00Z","timestamp":1461196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["419538\u20102012"],"award-info":[{"award-number":["419538\u20102012"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p>Bunyaviruses represent a growing threat to humans and livestock globally. The receptors, cellular factors and endocytic pathways used by these emerging pathogens to infect cells remain largely unidentified and poorly characterized. <jats:styled-content style=\"fixed-case\">DC\u2010SIGN<\/jats:styled-content> is a C\u2010type lectin highly expressed on dermal dendritic cells that has been found to act as an authentic entry receptor for many phleboviruses (<jats:italic>Bunyaviridae<\/jats:italic>), including Rift Valley fever virus (<jats:styled-content style=\"fixed-case\">RVFV<\/jats:styled-content>), Toscana virus (<jats:styled-content style=\"fixed-case\">TOSV<\/jats:styled-content>) and Uukuniemi virus (<jats:styled-content style=\"fixed-case\">UUKV<\/jats:styled-content>). We found that these phleboviruses can exploit another C\u2010type lectin, L\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content>, for infection. L\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content> shares 77% sequence homology with <jats:styled-content style=\"fixed-case\">DC\u2010SIGN<\/jats:styled-content> and is expressed on liver sinusoidal endothelial cells. L\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content> is required for <jats:styled-content style=\"fixed-case\">UUKV<\/jats:styled-content> binding but not for virus internalization. An endocytosis\u2010defective mutant of L\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content> was still able to mediate virus uptake and infection, indicating that L\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content> acts as an attachment receptor for phleboviruses rather than an endocytic receptor. Our results point out a fundamental difference in the use of the C\u2010type lectins L\u2010<jats:styled-content style=\"fixed-case\">SIGN<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DC\u2010SIGN<\/jats:styled-content> by <jats:styled-content style=\"fixed-case\">UUKV<\/jats:styled-content> to enter cells, although both proteins are closely related in terms of molecular structure and biological function. This study sheds new light on the molecular mechanisms by which phleboviruses target the liver and also highlights the added complexity in virus\u2013receptor interactions beyond attachment.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12393-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12393","type":"journal-article","created":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T01:03:47Z","timestamp":1458349427000},"page":"639-656","source":"Crossref","is-referenced-by-count":75,"title":["Differential Use of the C\u2010Type Lectins L\u2010<scp>SIGN<\/scp> and <scp>DC\u2010SIGN<\/scp> for Phlebovirus Endocytosis"],"prefix":"10.1111","volume":"17","author":[{"given":"Psylvia","family":"L\u00e9ger","sequence":"first","affiliation":[{"name":"CellNetworks \u2013 Cluster of Excellence and Department of Infectious Diseases, Virology University Hospital Heidelberg  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilou","family":"Tetard","sequence":"additional","affiliation":[{"name":"INRS\u2010Institut Armand\u2010Frappier  Laval Canada"},{"name":"Current address: Inserm UMR_S1134  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berthe","family":"Youness","sequence":"additional","affiliation":[{"name":"CellNetworks \u2013 Cluster of Excellence and Department of Infectious Diseases, Virology University Hospital Heidelberg  Heidelberg Germany"},{"name":"INRS\u2010Institut Armand\u2010Frappier  Laval Canada"},{"name":"Reproduction Genetics Unit, Department of Gynecological Endocrinology and Reproductive Medicine University Hospital  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Cordes","sequence":"additional","affiliation":[{"name":"CellNetworks \u2013 Cluster of Excellence and Department of Infectious Diseases, Virology University Hospital Heidelberg  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronan N.","family":"Rouxel","sequence":"additional","affiliation":[{"name":"INRS\u2010Institut Armand\u2010Frappier  Laval Canada"},{"name":"UR_0892 Unit\u00e9 de Virologie et Immunologie Mol\u00e9culaire INRA, CRJ  Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Flamand","sequence":"additional","affiliation":[{"name":"Structural Virology Institut Pasteur  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre\u2010Yves","family":"Lozach","sequence":"additional","affiliation":[{"name":"CellNetworks \u2013 Cluster of Excellence and Department of Infectious Diseases, Virology University Hospital Heidelberg  Heidelberg Germany"},{"name":"INRS\u2010Institut Armand\u2010Frappier  Laval Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2217\/fvl.15.52"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01191-08"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708738105"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.05.004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.05.007"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.06.007"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001623"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.015701-0"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.06.010"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2011.09.023"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02628-12"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001421"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/378747"},{"key":"e_1_2_6_15_1","first-page":"839","article-title":"Immunohistochemical and in situ localization of Crimean\u2010Congo hemorrhagic fever (CCHF) virus in human tissues and implications for CCHF pathogenesis","volume":"121","author":"Burt FJ","year":"1997","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002237"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.041053-0"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.18433\/J3N590"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb784"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104565200"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01893-13"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402296200"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.05.033"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301284200"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/v7062747"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.1.426"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12052"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.11.042"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.10.6152-6161.2005"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90709-X"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.14.7263-7275.2002"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.022418"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1778"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.03.012"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00260-1"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504337200"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.10.016"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03287-13"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.03.012"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.08.009"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003232"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.021204"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.11.001"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12899"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.6.2949-2957.2006"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90087-R"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(79)90006-3"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(73)90062-7"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-12-3053"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.2.257"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00388-14"}],"container-title":["Traffic"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftra.12393","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tra.12393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T16:45:54Z","timestamp":1694623554000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tra.12393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,21]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1111\/tra.12393"],"URL":"https:\/\/doi.org\/10.1111\/tra.12393","archive":["Portico"],"relation":{},"ISSN":["1398-9219","1600-0854"],"issn-type":[{"value":"1398-9219","type":"print"},{"value":"1600-0854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,21]]}}}