{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T08:56:40Z","timestamp":1790067400018,"version":"4.0.1"},"reference-count":62,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,10,5]],"date-time":"2016-10-05T00:00:00Z","timestamp":1475625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001692","name":"Croucher Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001692","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>Neurons rely heavily on axonal transport to deliver materials from the sites of synthesis to the axon terminals over distances that can be many centimetres long. <jats:styled-content style=\"fixed-case\">KIF1A<\/jats:styled-content> is the neuron\u2010specific kinesin with the fastest reported anterograde motor activity. Previous studies have shown that <jats:styled-content style=\"fixed-case\">KIF1A<\/jats:styled-content> transports a subset of synaptic proteins, neurofilaments and dense\u2010core vesicles. Using two\u2010colour live imaging, we showed that beta\u2010secretase 1 (<jats:styled-content style=\"fixed-case\">BACE1<\/jats:styled-content>)\u2010<jats:styled-content style=\"fixed-case\">mCherry<\/jats:styled-content> moves together with <jats:styled-content style=\"fixed-case\">KIF1A\u2010GFP<\/jats:styled-content> in both the anterograde and retrograde directions in superior cervical ganglions (<jats:styled-content style=\"fixed-case\">SCG<\/jats:styled-content>) neurons. We confirmed that <jats:styled-content style=\"fixed-case\">KIF1A<\/jats:styled-content> is functionally required for <jats:styled-content style=\"fixed-case\">BACE1<\/jats:styled-content> transport by using <jats:styled-content style=\"fixed-case\">KIF1A siRNA<\/jats:styled-content> and a <jats:styled-content style=\"fixed-case\">KIF1A<\/jats:styled-content> mutant construct (<jats:styled-content style=\"fixed-case\">KIF1A\u2010T312M<\/jats:styled-content>) to impair its motor activity. We further identified several cargoes that have little or no co\u2010migration with <jats:styled-content style=\"fixed-case\">KIF1A\u2010GFP<\/jats:styled-content> and also move independently from <jats:styled-content style=\"fixed-case\">BACE1\u2010mCherry<\/jats:styled-content>. Together, these findings support a primary role for <jats:styled-content style=\"fixed-case\">KIF1A<\/jats:styled-content> in the anterograde transport of <jats:styled-content style=\"fixed-case\">BACE1<\/jats:styled-content> and suggest that axonally transported cargoes are sorted into different classes of carrier vesicles in the cell body and are transported by cargo\u2010specific motor proteins through the axon.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12428-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12428","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T08:23:10Z","timestamp":1470212590000},"page":"1155-1167","source":"Crossref","is-referenced-by-count":32,"title":["<scp>KIF1A<\/scp> mediates axonal transport of <scp>BACE1<\/scp> and identification of independently moving cargoes in living <scp>SCG<\/scp> neurons"],"prefix":"10.1111","volume":"17","author":[{"given":"Christy O. Y.","family":"Hung","sequence":"first","affiliation":[{"name":"Department of Signalling Programme Babraham Institute  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Coleman","sequence":"additional","affiliation":[{"name":"Department of Signalling Programme Babraham Institute  Cambridge UK"},{"name":"John van Geest Centre for Brain Repair University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2774"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200212017"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-49522-3_4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.10.019"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.11.3717"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)00124-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00162-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.5.959"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200408113"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(00)00171-X"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.07.006"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000072"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2011.01.018"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211152200"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000300"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R800019200"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/414643a"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3089-04.2005"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2006.00434.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4188"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-013-1152-3"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4396-06.2007"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20934"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.44.31506"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90012-4"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970280108"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.9.2.333"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970230104"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2770-14.2014"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001539"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2010.09.007"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/35040102"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(03)01014-8"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1055"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.4161\/bioa.27049"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2014.09.018"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-9-1"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.31.061307.090711"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706825105"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3853"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E07-03-0261"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5039-03.2004"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.06.045"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.141.2.431"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2013-102182"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5221-12.2013"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1086\/344210"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10048-012-0324-y"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E09-04-0304"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201408056"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200407070"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1105681"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210392"},{"issue":"21","key":"e_1_2_6_55_1","doi-asserted-by":"crossref","first-page":"9287","DOI":"10.1523\/JNEUROSCI.22-21-09287.2002","article-title":"Insulin\u2010like growth factor\u2010I blocks Bcl\u20102 interacting mediator of cell death (Bim) induction and intrinsic death signaling in cerebellar granule neurons","volume":"22","author":"Linseman DA","year":"2002","journal-title":"J Neurosci"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00116-1"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e03-09-0704"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000300"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.20295"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-1-5"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/990114"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.24.1.677"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1177\/107385840200800408"}],"container-title":["Traffic"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftra.12428","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftra.12428","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tra.12428","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tra.12428","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tra.12428","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T00:22:19Z","timestamp":1696206139000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tra.12428"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,5]]},"references-count":62,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/tra.12428"],"URL":"https:\/\/doi.org\/10.1111\/tra.12428","archive":["Portico"],"relation":{},"ISSN":["1398-9219","1600-0854"],"issn-type":[{"value":"1398-9219","type":"print"},{"value":"1600-0854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,5]]}}}