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Consequently viral <jats:styled-content style=\"fixed-case\">DNAs<\/jats:styled-content> must be replicated, chromatinized and packed into progeny virions in infected cells. Although viral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> replication centers can be visualized by virtue of viral and cellular factors, the spatiotemporal regulation of viral genomes during subsequent steps remains to be elucidated. In this study, we used imaging analyses to examine the fate of adenoviral genomes and to track newly replicated viral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> as well as replication\u2010related factors. We show de novo formation of a subnuclear domain, which we termed Virus\u2010induced Post\u2010Replication (<jats:styled-content style=\"fixed-case\">ViPR<\/jats:styled-content>) body, that emerges concomitantly with or immediately after disintegration of initial replication centers. Using a nucleoside analogue, we show that viral genomes continue being synthesized in morphologically distinct replication compartments at the periphery of <jats:styled-content style=\"fixed-case\">ViPR<\/jats:styled-content> bodies and are then transported inward. In addition, we identified a nucleolar protein Mybbp1a as a molecular marker for <jats:styled-content style=\"fixed-case\">ViPR<\/jats:styled-content> bodies, which specifically associated with viral core protein <jats:styled-content style=\"fixed-case\">VII<\/jats:styled-content>. In conclusion, our work demonstrates the formation of previously uncharacterized viral <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> replication compartments specific for late phases of infection that produce progeny viral genomes accumulating in <jats:styled-content style=\"fixed-case\">ViPR<\/jats:styled-content> bodies.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12429-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12429","type":"journal-article","created":{"date-parts":[[2016,8,5]],"date-time":"2016-08-05T07:45:13Z","timestamp":1470383113000},"page":"1168-1180","source":"Crossref","is-referenced-by-count":27,"title":["Tracking adenovirus genomes identifies morphologically distinct late <scp>DNA<\/scp> replication compartments"],"prefix":"10.1111","volume":"17","author":[{"given":"Tetsuro","family":"Komatsu","sequence":"first","affiliation":[{"name":"Microbiologie 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