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We employed <jats:italic>Saccharomyces cerevisiae<\/jats:italic> to comprehensively study the impact of <jats:styled-content style=\"fixed-case\">FPs<\/jats:styled-content> on the toxicity of polyglutamine (<jats:styled-content style=\"fixed-case\">polyQ<\/jats:styled-content>) expansion proteins associated with Huntington's disease. The toxicity of <jats:styled-content style=\"fixed-case\">polyQ<\/jats:styled-content> fusion constructs is highly dependent on the sequences flanking the <jats:styled-content style=\"fixed-case\">polyQ<\/jats:styled-content> repeats. Thus, they represent a powerful tool to study the impact of fluorescent fusion partners. We observed significant differences on <jats:styled-content style=\"fixed-case\">polyQ<\/jats:styled-content> aggregation and toxicity between commonly used <jats:styled-content style=\"fixed-case\">FPs<\/jats:styled-content>. We generated a novel series of vectors with latest yeast\u2010optimized <jats:styled-content style=\"fixed-case\">FPs<\/jats:styled-content> for investigation of Htt toxicity, including a newly optimized blue <jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content> for expression in yeast. Our study highlights the importance of carefully choosing the optimal <jats:styled-content style=\"fixed-case\">FPs<\/jats:styled-content> when designing tagging strategies.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12453-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12453","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T23:08:32Z","timestamp":1476400112000},"page":"58-70","source":"Crossref","is-referenced-by-count":28,"title":["Polyglutamine toxicity in yeast uncovers phenotypic variations between different fluorescent protein fusions"],"prefix":"10.1111","volume":"18","author":[{"given":"Yuwei","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology The University of Western Ontario  London Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja E.","family":"Di 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