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Tail\u2010anchored (<jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>) proteins are integrated into organellar membranes via the C\u2010terminus, orienting the N\u2010terminus towards the cytosol. Localization of <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content> proteins occurs posttranslationally and is governed by the C\u2010terminus, which contains the integral transmembrane domain (<jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content>) and targeting sequence. Targeting of <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content> proteins is dependent on the hydrophobicity of the <jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content> as well as the length and composition of flanking amino acid sequences. We previously identified an unusual homologue of elongator protein, Elp3, in the apicomplexan parasite <jats:italic>Toxoplasma gondii<\/jats:italic> as a <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content> protein targeting the outer mitochondrial membrane. We sought to gain further insight into <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content> proteins and their targeting mechanisms using this early\u2010branching eukaryote as a model. Our bioinformatics analysis uncovered 59 predicted <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content> proteins in <jats:italic>Toxoplasma<\/jats:italic>, 9 of which were selected for follow\u2010up analyses based on representative features. We identified novel <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content> proteins that traffic to specific organelles in <jats:italic>Toxoplasma<\/jats:italic>, including the parasite endoplasmic reticulum, mitochondrion, and Golgi apparatus. Domain swap experiments elucidated that targeting of <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content> proteins to these specific organelles was strongly influenced by the <jats:styled-content style=\"fixed-case\">TMD<\/jats:styled-content> sequence, including charge of the flanking C\u2010terminal sequence.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12464-gra-0001.png\" xlink:title=\"image\" \/><\/jats:p>","DOI":"10.1111\/tra.12464","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T11:59:14Z","timestamp":1482148754000},"page":"149-158","source":"Crossref","is-referenced-by-count":15,"title":["Targeting of tail\u2010anchored membrane proteins to subcellular organelles in <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"18","author":[{"given":"Leah R.","family":"Padgett","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"Arrizabalaga","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Indiana University School of Medicine  Indianapolis Indiana"},{"name":"Department of Microbiology and Immunology Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"William J.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Indiana University School of Medicine  Indianapolis Indiana"},{"name":"Department of Microbiology and Immunology Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,17]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200303069"},{"issue":"3","key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/0962-8924(93)90066-A","article-title":"A class of membrane proteins with a C\u2010terminal anchor","volume":"3","author":"Kutay U","year":"1993","journal-title":"Trends Cell Biol"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.01-12-0570"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2016.02.010"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb06994.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600281"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2010.07.010"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.002410"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M804591200"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.143404"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.055970"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.06.025"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.707752"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920311798841717"},{"issue":"14","key":"e_1_2_5_16_1","doi-asserted-by":"crossref","first-page":"6483","DOI":"10.1016\/S0021-9258(18)50393-8","article-title":"The binding of cytochrome b5 to phospholipid vesicles and biological membranes. 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