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Reduced <jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content> coupling is associated with ageing, and disruptions of <jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content> coupling result in congenital myopathies for which there are few therapies. The precise localization of <jats:styled-content style=\"fixed-case\">DHPRs<\/jats:styled-content> to triads is critical for <jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content> coupling, yet trafficking of the <jats:styled-content style=\"fixed-case\">DHPR<\/jats:styled-content> to triads is not well understood. Using dynamic imaging of zebrafish muscle fibers, we find that <jats:styled-content style=\"fixed-case\">DHPR<\/jats:styled-content> is transported along the longitudinal <jats:styled-content style=\"fixed-case\">SR<\/jats:styled-content> in a microtubule\u2010independent mechanism. Furthermore, transport of <jats:styled-content style=\"fixed-case\">DHPR<\/jats:styled-content> in the <jats:styled-content style=\"fixed-case\">SR<\/jats:styled-content> membrane is differentially affected in null mutants of Stac3 or <jats:styled-content style=\"fixed-case\">DHPR\u03b2<\/jats:styled-content>, two essential components of <jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content> coupling. These findings reveal previously unappreciated features of <jats:styled-content style=\"fixed-case\">DHPR<\/jats:styled-content> motility within the <jats:styled-content style=\"fixed-case\">SR<\/jats:styled-content> prior to assembly at triads.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12502-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12502","type":"journal-article","created":{"date-parts":[[2017,7,11]],"date-time":"2017-07-11T16:02:37Z","timestamp":1499788957000},"page":"622-632","source":"Crossref","is-referenced-by-count":12,"title":["Transport of the alpha subunit of the voltage gated L\u2010type calcium channel through the sarcoplasmic reticulum occurs prior to localization to triads and requires the beta subunit but not Stac3 in skeletal muscles"],"prefix":"10.1111","volume":"18","author":[{"given":"Jeremy W.","family":"Linsley","sequence":"first","affiliation":[{"name":"Cell and 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