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Peroxisome movement and positioning allows interaction with other organelles and is crucial for their cellular function. In mammalian cells, such movement is microtubule\u2010dependent and mediated by kinesin and dynein motors. The mechanisms of motor recruitment to peroxisomes are largely unknown, as well as the role this plays in peroxisome membrane dynamics and proliferation. Here, using a combination of microscopy, live\u2010cell imaging analysis and mathematical modelling, we identify a role for Mitochondrial Rho GTPase 1 (MIRO1) as an adaptor for microtubule\u2010dependent peroxisome motility in mammalian cells. We show that MIRO1 is targeted to peroxisomes and alters their distribution and motility. Using a peroxisome\u2010targeted MIRO1 fusion protein, we demonstrate that MIRO1\u2010mediated pulling forces contribute to peroxisome membrane elongation and proliferation in cellular models of peroxisome disease. Our findings reveal a molecular mechanism for establishing peroxisome\u2010motor protein associations in mammalian cells and provide new insights into peroxisome membrane dynamics in health and disease.<\/jats:p>\n                  <jats:p>\n                    <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12549-gra-0001.png\" xlink:title=\"image\"\/>\n                  <\/jats:p>","DOI":"10.1111\/tra.12549","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T15:00:40Z","timestamp":1516806040000},"page":"229-242","source":"Crossref","is-referenced-by-count":92,"title":["A role for Mitochondrial Rho GTPase 1 (MIRO1) in motility and membrane dynamics of peroxisomes"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4669-985X","authenticated-orcid":false,"given":"In\u00eas G.","family":"Castro","sequence":"first","affiliation":[{"name":"Biosciences University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Richards","sequence":"additional","affiliation":[{"name":"LSI University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8099-8776","authenticated-orcid":false,"given":"Jeremy","family":"Metz","sequence":"additional","affiliation":[{"name":"Biosciences University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2867-1864","authenticated-orcid":false,"given":"Joseph L.","family":"Costello","sequence":"additional","affiliation":[{"name":"Biosciences University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7791-3439","authenticated-orcid":false,"given":"Josiah B.","family":"Passmore","sequence":"additional","affiliation":[{"name":"Biosciences University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina A.","family":"Schrader","sequence":"additional","affiliation":[{"name":"Biosciences University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Gouveia","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Aveiro  Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3397-0460","authenticated-orcid":false,"given":"Daniela","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Aveiro  Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2146-0535","authenticated-orcid":false,"given":"Michael","family":"Schrader","sequence":"additional","affiliation":[{"name":"Biosciences University of Exeter  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2015.09.024"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2015.11.015"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2013.07.026"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2015.00056"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2015.09.016"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22369"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2960"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-1788-0_21"},{"key":"e_1_2_7_10_1","first-page":"24","article-title":"Interaction of microtubules with peroxisomes. 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