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Conserved endocytic components and plant\u2010like features suggest <jats:italic>T. gondii<\/jats:italic> endocytic trafficking involves transit through early and late endosome\u2010like compartments (ELCs) and potentially the trans\u2010Golgi network (TGN) as in plants. However, exocytic trafficking to regulated secretory organelles, micronemes and rhoptries, also proceeds through ELCs and requires classical endocytic components, including a dynamin\u2010related protein, DrpB. Here, we show that host cytosolic proteins are endocytosed within 7 minutes post\u2010invasion, trafficked through ELCs en route to the VAC, and degraded within 30\u2009minutes. We could not definitively interpret if ingested protein is trafficked through the TGN. We also found that parasites ingest material from the host cytosol throughout the parasite cell cycle. Ingested host proteins colocalize with immature microneme proteins, proM2AP and proMIC5, in transit to the micronemes, but not with the immature rhoptry protein proRON4, indicating that endocytic trafficking of ingested protein intersects with exocytic trafficking of microneme proteins. Finally, we show that conditional expression of a DrpB dominant negative mutant increases <jats:italic>T. gondii<\/jats:italic> ingestion of host\u2010derived proteins, suggesting that DrpB is not required for parasite endocytosis.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12556-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12556","type":"journal-article","created":{"date-parts":[[2018,2,13]],"date-time":"2018-02-13T15:49:26Z","timestamp":1518536966000},"page":"336-353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["Intersection of endocytic and exocytic systems in <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"19","author":[{"given":"Olivia L.","family":"McGovern","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology University of Michigan School of Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Rivera\u2010Cuevas","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Michigan School of Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geetha","family":"Kannan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Michigan School of Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Andrew J.","family":"Narwold","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Michigan School of Medicine  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6859-8895","authenticated-orcid":false,"given":"Vern 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