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However, it is presently unknown if TRiC also has any effect on axonal transport in wild\u2010type neurons. Here, we examined how TRiC impacted the retrograde axonal transport of brain\u2010derived neurotrophic factor (BDNF). We found that expression of a single TRiC subunit significantly enhanced axonal transport of BDNF, leading to an increase in instantaneous velocity with a concomitant decrease in pauses for retrograde BDNF transport. The transport enhancing effect by TRiC was dependent on endogenous tau expression because no effect was seen in neurons from tau knockout mice. We showed that TRiC regulated the level of cyclin\u2010dependent kinase 5 (CDK5)\/p35 positively, contributing to TRiC\u2010mediated tau phosphorylation (ptau). Expression of a single TRiC subunit increased the level of ptau while downregulation of the TRiC complex decreased ptau. We further demonstrated that TRiC\u2010mediated increase in ptau induced detachment of tau from microtubules. Our study has thus revealed that TRiC\u2010mediated increase in tau phosphorylation impacts retrograde axonal transport.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tra12610-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1111\/tra.12610","type":"journal-article","created":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T03:10:06Z","timestamp":1534561806000},"page":"840-853","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["T\u2010complex protein 1\u2010ring complex enhances retrograde axonal transport by modulating tau phosphorylation"],"prefix":"10.1111","volume":"19","author":[{"given":"Xu\u2010Qiao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurosciences University of California San Diego  La Jolla California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of California San Diego  La Jolla California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jazmin B.","family":"Florio","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of California San Diego  La Jolla California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Rockenstein","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of California San Diego  La Jolla California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliezer","family":"Masliah","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of California San Diego  La Jolla California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Mobley","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of California San Diego  La Jolla California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Rissman","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of California San Diego  La Jolla California"},{"name":"Veterans Affairs San Diego Healthcare System  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3660-088X","authenticated-orcid":false,"given":"Chengbiao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Neurosciences University of California San Diego  La Jolla California"},{"name":"Veterans Affairs San Diego Healthcare System  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.04.013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2011.05.003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10317"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2004.09.015"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1477"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1478"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222663110"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1603020113"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13649"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.08.017"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40859"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102985"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152993"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200108057"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2003.04.007"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2006-9S327"},{"key":"e_1_2_7_18_1","unstructured":"KosikKS.Tau\u2010er of Power. 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