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In mammals, mutations in AP\u20103 cause Hermansky\u2010Pudlak syndrome type 2, cyclic neutropenias, and a form of epileptic encephalopathy. In budding yeast, AP\u20103 carries cargo directly from the trans\u2010Golgi to the lysosomal vacuole. Despite the pathway's importance and its discovery two decades ago, rapid screens and selections for AP\u20103 mutants have not been available. We now report GNSI, a synthetic, genetically encoded reporter that allows rapid plate\u2010based assessment of AP\u20103 functional deficiency, using either chromogenic or growth phenotype readouts. This system identifies defects in both the formation and consumption of AP\u20103 carrier vesicles and is adaptable to high\u2010throughput screening or selection in both plate array and liquid batch culture formats. Episomal and integrating plasmids encoding GNSI have been submitted to the Addgene repository.<\/jats:p>","DOI":"10.1111\/tra.12772","type":"journal-article","created":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T06:35:04Z","timestamp":1606113304000},"page":"38-44","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genetically encoded multimode reporter of adaptor complex 3 traffic in budding yeast"],"prefix":"10.1111","volume":"22","author":[{"given":"Rachael L.","family":"Plemel","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Washington  Seattle Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg","family":"Odorizzi","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular, and Developmental Biology University of Colorado  Boulder 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