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Particularly when it comes to studying phagocytosis and phagosomal maturation\u2014a signature cellular process of the macrophage\u2014many commonly used cell lines are not representative of what occurs in primary macrophages. A system developed by Mark Kamps' group, that utilizes conditionally constitutive activity of <jats:italic>Hox<\/jats:italic> transcription factors (<jats:italic>Hoxb8<\/jats:italic> and <jats:italic>Hoxa9<\/jats:italic>) to immortalize differentiation\u2010competent myeloid cell progenitors of mice, offers an alternative to the macrophage\/macrophage\u2010like dichotomy. In this resource, we will review the use of <jats:italic>Hoxb8<\/jats:italic> and <jats:italic>Hoxa9<\/jats:italic> as hematopoietic regulators to conditionally immortalize murine hematopoietic progenitor cells which retain their ability to differentiate into many functional immune cell types including macrophages, neutrophils, basophils, osteoclasts, eosinophils, dendritic cells, as well as limited potential for the generation of lymphocytes. We further demonstrate that the use of macrophages derived from <jats:italic>Hoxb8<\/jats:italic>\/<jats:italic>Hoxa9<\/jats:italic> immortalized progenitors and their similarities to bone marrow\u2010derived macrophages. To supplement the existing data, mass spectrometry\u2010based proteomics, flow cytometry, cytology, and in vitro phagosomal assays were conducted on macrophages derived from <jats:italic>Hoxb8<\/jats:italic> immortalized progenitors and compared to bone marrow\u2010derived macrophages and the macrophage\u2010like cell line J774. We additionally propose the use of a standardized nomenclature to describe cells derived from the <jats:italic>Hoxb8<\/jats:italic>\/<jats:italic>Hoxa9<\/jats:italic> system in anticipation of their expanded use in the study of leukocyte cell biology.<\/jats:p>","DOI":"10.1111\/tra.12869","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T02:20:34Z","timestamp":1663554034000},"page":"538-553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Hox<\/i>\u2010driven conditional immortalization of myeloid and lymphoid progenitors: Uses, advantages, and future potential"],"prefix":"10.1111","volume":"23","author":[{"given":"Shranjit S.","family":"Lail","sequence":"first","affiliation":[{"name":"Department of Medical Science, Cumming School of Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6552-6650","authenticated-orcid":false,"given":"Corey R.","family":"Arnold","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Cumming School of Medicine University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz G. 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