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The toll\u2010like receptor (TLR) 2 pathway utilizes the trafficking molecule adaptor protein 3 (AP\u20103) to activate interleukin (IL)\u20106 signaling from within phagosomal compartments. To better understand the vesicular pathways that may contribute to intracellular signaling and cooperate with AP\u20103, we performed a vesicular siRNA screen. We identified Rab8 and Rab11 GTPases as important in IL\u20106 induction upon stimulation with the TLR2 ligand Pam<jats:sub>3<\/jats:sub>CSK<jats:sub>4<\/jats:sub> or the pathogen, <jats:italic>Borrelia burgdorferi<\/jats:italic> (<jats:italic>Bb<\/jats:italic>), the causative agent of Lyme disease. These Rabs were recruited to late and lysosomal stage phagosomes and co\u2010transported with TLR2 signaling adaptors and effectors, such as MyD88, TRAM and TAK1, in an AP\u20103\u2010dependent manner. Our data support a model where AP\u20103 mediates the recruitment of recycling and secretory vesicles and the assembly of signaling complexes at the phagosome.<\/jats:p>","DOI":"10.1111\/tra.12870","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T01:44:07Z","timestamp":1665625447000},"page":"558-567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An <scp>AP<\/scp>\u20103\u2010dependent pathway directs phagosome fusion with Rab8 and Rab11 vesicles involved in <scp>TLR2<\/scp> signaling"],"prefix":"10.1111","volume":"23","author":[{"given":"Tanja","family":"Petnicki\u2010Ocwieja","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Microbiology Tufts University School of Medicine  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bijaya","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department 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