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Genetic studies predicted a role for Arl15 in type\u20102 diabetes, insulin resistance, adiposity, and rheumatoid arthritis. Cell biological studies implicated Arl15 in regulating various cellular processes, including magnesium homeostasis and TGF\u03b2 signaling. However, the role of Arl15 in vesicular transport is poorly defined. We evaluated the function of Arl15 using techniques to quantify cargo trafficking to mechanobiology. Fluorescence microscopy of stably expressing Arl15\u2010GFP HeLa cells showed its localization primarily to the Golgi and cell surface. The depletion of Arl15 causes the mislocalization of selective Golgi cargo, such as caveolin\u20102 and STX6, in the cells. Consistently, expression of GTPase\u2010independent dominant negative mutants of Arl15 (Arl15\n                    <jats:sup>V80A,A86L,E122K<\/jats:sup>\n                    and Arl15\n                    <jats:sup>C22Y,C23Y<\/jats:sup>\n                    ) results in mislocalization of caveolin\u20102 and STX6 from the Golgi. However, the localization of Arl15 to the Golgi is dependent on its palmitoylation and Arf1\u2010dependent Golgi integrity. At the cellular level, Arl15 depleted cells display enhanced cell spreading and adhesion strength. Traction force microscopy experiments revealed that Arl15 depleted cells exert higher tractions and generate multiple focal adhesion points during the initial phase of cell adhesion compared to control cells. Collectively, these studies implicate a functional role for Arl15 in regulating cargo transport from the Golgi to regulate cell surface processes.\n                  <\/jats:p>","DOI":"10.1111\/tra.70004","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T01:34:54Z","timestamp":1744853694000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Golgi Localized Arl15 Regulates Cargo Transport and Cell Adhesion"],"prefix":"10.1111","volume":"26","author":[{"given":"Prerna","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5958-6884","authenticated-orcid":false,"given":"Pooja","family":"Hoovina Venkatesh","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shalini","family":"Samal","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology Indian Institute of Science  Bangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Paddillaya","sequence":"additional","affiliation":[{"name":"Centre for Biosystems Science and Engineering Indian Institute of Science  Bangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Biology Indian Institute of Science Education and Research  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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