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In\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    , recent studies revealed that the Kelch domain\u2010containing protein 13 and AP\u20102 participate in hemoglobin uptake via cytostomes. However, clathrins appear not to be involved in this process because they primarily associate with AP\u20101. To investigate the roles of clathrins in\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    , we characterized the clathrin heavy chain (\n                    <jats:italic>Pf<\/jats:italic>\n                    CHC), the clathrin light chain (\n                    <jats:italic>Pf<\/jats:italic>\n                    CLC), and the AP\u20101 \u03b3 subunit (\n                    <jats:italic>Pf<\/jats:italic>\n                    AP\u20101 \u03b3). Extensive interactome analyses confirmed the major association of clathrins with AP\u20101 components alongside proteins involved in cytostome formation. Live\u2010cell imaging and protein colocalization studies showed that\n                    <jats:italic>Pf<\/jats:italic>\n                    CHC,\n                    <jats:italic>Pf<\/jats:italic>\n                    CLC, and\n                    <jats:italic>Pf<\/jats:italic>\n                    AP\u20101 \u03b3 are localized in the parasite cytoplasm, predominantly at the parasite periphery and near the\n                    <jats:italic>cis<\/jats:italic>\n                    \u2010Golgi. Ultrastructural studies using ascorbate peroxidase 2\u2010based electron microscopy confirmed their presence at coated vesicle\u2010like structures at the parasite periphery and, unexpectedly, at the collars of cytostomes. Knockdown of\n                    <jats:italic>Pf<\/jats:italic>\n                    CHC led to the formation of abnormally long cytostome tubes and impaired hemoglobin uptake, suggesting that clathrins are involved in cytostome processes in\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/tra.70025","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T12:26:01Z","timestamp":1766751961000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clathrins Are Involved in the Endocytosis of Host Cytosol in the Malaria Parasite"],"prefix":"10.1111","volume":"27","author":[{"given":"Jun","family":"Miao","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Morsani College of Medicine, University of South Florida  Tampa Florida USA"},{"name":"Center for Global Health and Infectious Diseases Research College of Public Health, University of South Florida  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6779-1888","authenticated-orcid":false,"given":"Amuza Byaruhanga","family":"Lucky","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Morsani College of Medicine, University of South Florida  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Ning","sequence":"additional","affiliation":[{"name":"Huck Institute of Life Sciences, the Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Microbiology Immunology &amp; Molecular Genetics, University of California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Morsani College of Medicine, University of South Florida  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faiza","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Morsani College of Medicine, University of South Florida  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Morsani College of Medicine, University of South Florida  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Global Health and Infectious Diseases Research College of Public Health, University of South Florida  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Morsani College of Medicine, University of South Florida  Tampa Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. 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