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KIF13B, a kinesin 3 family cellular motor, regulates VEGF\/VEGFR2 signaling by bringing VEGFR2 to the cell surface. Regulating the trafficking of VEGFR2 could be a potential therapeutic target in pathogenesis originating from abnormal angiogenesis. However, the regulation mechanism of VEGFR2 trafficking is not fully understood. Here, we show that protein kinase D (PKD) phosphorylates KIF13B. The phosphorylation of KIF13B by PKD is required for KIF13B\u2010VEGFR2 interaction and KIF13B\u2010microtubule interaction. Live cell imaging using VEGFR2\u2010mCherry revealed that the PKD inhibitor reduced the mean speed and total distance of VEGFR2 vesicular trafficking. Finally, inhibition of PKD also mitigated VEGF\u2010induced permeability measured by trans\u2010endothelial electrical resistance (TEER). Our results demonstrate how VEGFR2 trafficking is regulated by the phosphorylation of kinesin motor KIF13B by protein kinase PKD. We anticipate that elucidating the underlying mechanism of VEGFR2 trafficking can help progress the therapy development of the target diseases related to abnormal angiogenesis.<\/jats:p>","DOI":"10.1111\/tra.70032","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T05:26:33Z","timestamp":1770960393000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protein Kinase D Regulates Kinesin\u2010Mediated\n                    <scp>VEGFR2<\/scp>\n                    Trafficking and Influences Retinal Endothelial Permeability"],"prefix":"10.1111","volume":"27","author":[{"given":"Benedict Ryungrae","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Regenerative Medicine University of Illinois College of Medicine  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun\u2010Dong","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Regenerative Medicine University of Illinois College of Medicine  Chicago Illinois USA"},{"name":"Department of Food and Nutrition Sunchon National University  Sunchon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yudai","family":"Kudo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Regenerative Medicine University of Illinois College of Medicine  Chicago Illinois USA"},{"name":"The United Graduate School of Drug Discovery and Medical Information Sciences Gifu University  Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirushi","family":"Gunasekara","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Liberal Arts and Sciences University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geyi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Regenerative Medicine University of Illinois College of Medicine  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayeman","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Regenerative Medicine University of Illinois College of Medicine  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen B.","family":"Waters","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Regenerative Medicine University of Illinois College of Medicine  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying S.","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Liberal Arts and Sciences University of Illinois Chicago  Chicago Illinois USA"},{"name":"Department of Biomedical Engineering, Colleges of Engineering and Medicine University of Illinois Chicago  Chicago Illinois USA"},{"name":"Department of Biochemistry and Molecular Genetics, College of Medicine University of Illinois Chicago  Chicago Illinois USA"},{"name":"University of Illinois Cancer Center  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaori H.","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Regenerative Medicine University of Illinois College of Medicine  Chicago Illinois USA"},{"name":"University of Illinois Cancer Center  Chicago Illinois USA"},{"name":"Department of Ophthalmology and Visual Sciences University of Illinois College of Medicine  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000517520"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.81.2.154"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2008.05.001"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0000000000000385"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S115121"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2014.64"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep29619"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-06-291690"},{"key":"e_1_2_12_10_1","first-page":"1404","article-title":"Ubiquitination of Basal VEGFR2 Regulates Signal Transduction and Endothelial Function","volume":"6","author":"Smith G. 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